The P4-type ATPase ATP11C is essential for B lymphopoiesis in adult bone marrow
| Autor: | David Nemazee, Pei Lin, Owen M. Siggs, Bruce Beutler, Elaine Pirie, Christoph Huber, Yu Xia, Carrie N. Arnold |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Genotype ATPase Immunology Mice Transgenic Context (language use) Article Immunophenotyping Mice Bone Marrow B cell development medicine Animals Immunology and Allergy Lymphopoiesis Progenitor cell B cell Adenosine Triphosphatases B-Lymphocytes Mice Inbred BALB C Mice Inbred C3H Fetus IL-7 biology Reverse Transcriptase Polymerase Chain Reaction Interleukin-7 Gene Expression Regulation Developmental flippase Molecular biology Cell biology Mice Inbred C57BL medicine.anatomical_structure Immunoglobulin M Trans-Activators biology.protein RNA Female Bone marrow fetal liver |
| Zdroj: | Nature immunology |
| ISSN: | 1529-2916 1529-2908 |
| Popis: | B lymphopoiesis begins in the fetal liver, switching after birth to the bone marrow, where it persists for life. The unique developmental outcomes of each phase are well documented, yet their molecular requirements are not. Here we describe two allelic X-linked mutations in mice that caused cell-intrinsic arrest of adult B lymphopoiesis. Mutant fetal liver progenitors generated B cells in situ but not in irradiated adult bone marrow, which emphasizes a necessity for the affected pathway only in the context of adult bone marrow. The causative mutations were ascribed to Atp11c, which encodes a P4-type ATPase with no previously described function. Our data establish an essential, cell-autonomous and context-sensitive function for ATP11C, a putative aminophospholipid flippase, in B cell development. |
| Databáze: | OpenAIRE |
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