Electrochemical Biosensor for Markers of Neurological Esterase Inhibition
| Autor: | Paul Satoh, Robert M. Worden, Neda Rafat |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Working electrode
organophosphate Diffusion design Clinical Biochemistry Kinetics Biosensing Techniques macromolecular substances 02 engineering and technology Electrochemistry 01 natural sciences Article amperometric biosensor Chemical kinetics neural esterase dimensionless Electrodes Phenylacetates Chromatography Monophenol Monooxygenase Chemistry 010401 analytical chemistry technology industry and agriculture Electrochemical Techniques acetylcholinesterase General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology inhibition 0104 chemical sciences Phenylacetate Electrode flux control Cholinesterase Inhibitors 0210 nano-technology optimization Biosensor mathematical model TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors, Vol 11, Iss 459, p 459 (2021) Biosensors Volume 11 Issue 11 |
| ISSN: | 2079-6374 |
| Popis: | A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working electrode of a screen-printed electrode (SPE) array. The reaction chemistry included a redox-recycle amplification mechanism to improve the biosensor’s current output and sensitivity. A mechanistic mathematical model of the biosensor was used to simulate key diffusion and reaction steps, including diffusion of AChE’s reactant (phenylacetate) and inhibitor, the reaction kinetics of the two enzymes, and electrochemical reaction kinetics at the SPE’s working electrode. The model was validated by showing that it could reproduce a steady-state biosensor current as a function of the inhibitor (PMSF) concentration and unsteady-state dynamics of the biosensor current following the addition of a reactant (phenylacetate) and inhibitor phenylmethylsulfonylfluoride). The model’s utility for characterizing and optimizing biosensor performance was then demonstrated. It was used to calculate the sensitivity of the biosensor’s current output and the redox-recycle amplification factor as a function of experimental variables. It was used to calculate dimensionless Damkohler numbers and current-control coefficients that indicated the degree to which individual diffusion and reaction steps limited the biosensor’s output current. Finally, the model’s utility in designing IBE biosensors and operating conditions that achieve specific performance criteria was discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|