Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU
| Autor: | Frank Kunst, R. Dedonder, D Henner, Tarek Msadek, A Klier, Georges Rapoport |
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| Přispěvatelé: | Biochimie Microbienne, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Genentech, Inc. [San Francisco], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1990 |
| Předmět: |
MESH: Signal Transduction
genetic structures Hydrolases Operon MESH: Genes Regulator [SDV]Life Sciences [q-bio] Catabolite repression MESH: Amino Acid Sequence Bacillus subtilis MESH: Base Sequence MESH: Genotype Genes Regulator Cloning Molecular Promoter Regions Genetic Peptide sequence Regulator gene chemistry.chemical_classification Regulation of gene expression MESH: Gene Expression Regulation Bacterial 0303 health sciences MESH: Codon biology MESH: Escherichia coli RNA-Directed DNA Polymerase MESH: Hydrolases Amino acid Phenotype [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry Research Article Plasmids Rhizobium Signal Transduction MESH: Rhizobium MESH: Operon MESH: Mutation Genotype Molecular Sequence Data MESH: Phenotype MESH: Sequence Homology Nucleic Acid Microbiology 03 medical and health sciences MESH: RNA-Directed DNA Polymerase MESH: Plasmids Sequence Homology Nucleic Acid MESH: Promoter Regions Genetic Escherichia coli MESH: Cloning Molecular Amino Acid Sequence RNA Messenger Codon Molecular Biology Gene MESH: RNA Messenger 030304 developmental biology MESH: Molecular Sequence Data Base Sequence 030306 microbiology Gene Expression Regulation Bacterial MESH: Bacillus subtilis biology.organism_classification chemistry Mutation bacteria |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1990, 172 (2), pp.824-834. ⟨10.1128/jb.172.2.824-834.1990⟩ Journal of Bacteriology, 1990, 172 (2), pp.824-834. ⟨10.1128/jb.172.2.824-834.1990⟩ |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.172.2.824-834.1990 |
| Popis: | International audience; The rates of synthesis of a class of both secreted and intracellular degradative enzymes in Bacillus subtilis are controlled by a signal transduction pathway defined by at least four regulatory genes: degS, degU, degQ (formerly sacQ), and degR (formerly prtR). The DegS-DegU proteins show amino acid similarities with two-component procaryotic modulator-effector pairs such as NtrB-NtrC, CheA-CheY, and EnvZ-OmpR. By analogy with these systems, it is possible that DegS is a protein kinase which could catalyze the transfer of a phosphoryl moiety to DegU, which acts as a positive regulator. DegR and DegQ correspond to polypeptides of 60 and 46 amino acids, respectively, which also activate the synthesis of degradative enzymes. We show that the degS and degU genes are organized in an operon. The putative sigma A promoter of the operon was mapped upstream from degS. Mutations in degS and degU were characterized at the molecular level, and their effects on transformability and cell motility were studied. The expression of degQ was shown to be subject both to catabolite repression and DegS-DegU-mediated control, allowing an increase in the rate of synthesis of degQ under conditions of nitrogen starvation. These results are consistent with the hypothesis that this control system responds to an environmental signal such as limitations of nitrogen, carbon, or phosphate sources. |
| Databáze: | OpenAIRE |
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