Bioactive constituents from Medicago sativa L. with antioxidant, neuroprotective and acetylcholinesterase inhibitory activities
| Autor: | Mengchao Lv, Danqi Li, Lixin Zhang, Changfeng Liu, Liu Xuegui, Guo-Dong Yao, Ming-Yuan Huang, Yuqiu Sun, Pin-Yi Gao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
DPPH Aché medicine.medical_treatment Isoflavane glycosides Medicine (miscellaneous) 01 natural sciences Neuroprotection Medicago sativa L AChE inhibitory activity chemistry.chemical_compound Antioxidant activity Neuroprotective activity medicine TX341-641 Medicago sativa chemistry.chemical_classification Nutrition and Dietetics ABTS 010405 organic chemistry Nutrition. Foods and food supply Glycoside Acetylcholinesterase language.human_language 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Biochemistry language Food Science |
| Zdroj: | Journal of Functional Foods, Vol 45, Iss, Pp 371-380 (2018) |
| ISSN: | 1756-4646 |
| Popis: | Medicago sativa L. is an important source of functional foods and supplements, which can improve human health. The present work was carried out to search for potential antioxidants from M. sativa to treat neurodegeneration. Four new isoflavane glycosides (1–4) were isolated from M. sativa, along with seven known compounds (5–11). Their structures including absolute configurations were elucidated by multi-spectroscopic data analyses, chemical methods, and quantum chemical ECD calculations. The compounds (1–11) were tested for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and Ferric reducing antioxidant power (FRAP) assays. All the isolated compounds displayed different degrees of antioxidant effects. Meanwhile, some isolates also possessed acetylcholinesterase (AChE) inhibitory activities. A possible mechanism of interaction between the active compounds and AChE was also investigated by molecular docking studies. Furthermore, compounds (1–6 and 10–11) exhibited potent neuroprotective effects against H2O2-induced cellular damage in human neuroblastoma SH-SY5Y cells. |
| Databáze: | OpenAIRE |
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