Renal expression of endothelial and inducible nitric oxide synthase, and formation of peroxynitrite-modified proteins and reactive oxygen species in Wegener's granulomatosis

Autor: Peter Heeringa, Han Moshage, Cees G. M. Kallenberg, Gerard Dijkstra, Jan Willem Cohen Tervaert, Anton T. M. G. Tiebosch, Harry van Goor, Alie de Jager-Krikken, Andre Zandvoort, Marc Bijl
Přispěvatelé: Groningen Kidney Center (GKC), Translational Immunology Groningen (TRIGR), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Groningen Institute for Organ Transplantation (GIOT), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Rok vydání: 2001
Předmět:
Adult
Male
CD31
Pathology
medicine.medical_specialty
BLOOD
Nitric Oxide Synthase Type III
Blotting
Western
Lipopolysaccharide Receptors
Lewis X Antigen
Nitric Oxide Synthase Type II
3
3'-Diaminobenzidine
Inflammation
Kidney
nitric oxide synthases
CLASSIFICATION
Pathology and Forensic Medicine
Nitric oxide
ACTIVATION
chemistry.chemical_compound
Enos
oxygen radicals
medicine
Humans
Aged
nitrotyrosine
biology
Nitrotyrosine
Granulomatosis with Polyangiitis
Hydrogen Peroxide
RAT NEPHROTOXIC NEPHRITIS
Middle Aged
biology.organism_classification
Wegener's granulomatosis
Platelet Endothelial Cell Adhesion Molecule-1
Endothelial stem cell
Nitric oxide synthase
MICE
medicine.anatomical_structure
chemistry
biology.protein
Tyrosine
Female
Nitric Oxide Synthase
medicine.symptom
Reactive Oxygen Species
CRESCENTIC GLOMERULONEPHRITIS
Zdroj: JOURNAL OF PATHOLOGY, 193(2), 224-232. Wiley
ISSN: 1096-9896
0022-3417
Popis: To investigate the role of nitric oxide (NO) in glomerular inflammation, the expression of endothelial NO synthase (eNOS) and inducible NOS (iNOS) was studied in conjunction with inflammatory cell influx, H2O2 production, and the formation of nitrotyrosines in renal biopsies from patients with Wegener's granulomatosis (WG), Renal cryostat sections from patients with WG (n = 15) were stained by immunohistochemistry for eNOS, iNOS, endothelial cells (CD31), nitrotyrosines, polymorphonuclear cells (PMNs, CD15), and monocytes/macrophages (CD14, CD68), Production of H2O2 was identified by enzyme cytochemistry using diaminobenzidine. In control tissues, strong staining for eNOS was found in glomerular and interstitial tubular capillaries and cortical vessels. A significant reduction in eNOS expression was found in WG biopsies, which was associated with a reduction in CD31 expression. Expression of iNOS was found in infiltrating inflammatory cells, mainly located in the interstitium, H2O2-producing cells were detected in glomeruli and were abundantly present in the interstitium. Nitrotyrosine-positive cells, however, were almost exclusively found in the interstitium, It is concluded that renal inflammation in WG is associated with the induction of iNOS in inflammatory cells and the formation of nitrotyrosines, Expression of eNOS in glomerular capillaries is lost, most likely due to endothelial cell damage. These results suggest that decreased NO. production by endothelial cells, in conjunction with increased NO. production by iNOS-positive inflammatory cells, is involved in renal tissue injury in WG, Copyright (C) 2000 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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