TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions
| Autor: | Marta Irigoyen, Juan J. Martinez-Irujo, E. Salvo, Elena Ansó, J. Dotor de las Herrerías, Ana Rouzaut |
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| Rok vydání: | 2008 |
| Předmět: |
Integrins
government.form_of_government Integrin Extracellular matrix Cellular and Molecular Neuroscience Laminin Cell Movement Transforming Growth Factor beta Cell Adhesion Humans Cell adhesion Molecular Biology Cells Cultured DNA Primers Pharmacology Extracellular Matrix Proteins biology Base Sequence fungi Endothelial Cells Cell Biology Molecular biology eye diseases Cell Hypoxia Cell biology Extracellular Matrix Up-Regulation Endothelial stem cell Fibronectin Lymphatic Endothelium biology.protein government Molecular Medicine sense organs TGFBI |
| Zdroj: | Cellular and molecular life sciences : CMLS. 65(14) |
| ISSN: | 1420-682X |
| Popis: | TGFbeta-induced protein (TGFBI) is an extracellular protein that mediates cell adhesion to collagen, laminin and fibronectin through its interaction with different beta integrins. We had previously reported that hypoxia-induced TGFBI mRNA expression in lymphatic endothelial cells (LEC). Here, we demonstrate that TGFBI can contribute to hypoxia-induced increases in LEC adhesion to the ECM. We show that while there are no changes in alpha1, alpha4, alphav, beta1, beta2, beta3, alpha5beta1, alphavbeta3, alphavbeta5 integrin expression on the LEC surface after hypoxia exposure, there exists an accumulation of TGFBI adaptor protein in LEC supernatants. We also demonstrate that hypoxia driven TGBFI expression is dependent on TGFbeta production by LEC. Furthermore, we show that TGFBI mediated LEC adhesion and migration through the ECM by its binding to the beta3 integrin. The identification of the specific mechanisms regulating LEC-ECM interactions may help us design new therapeutic applications for diseases in which lymphatic vessel function is compromised. |
| Databáze: | OpenAIRE |
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