Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation
| Autor: | Mitsutoshi Oguri, Hideki Horibe, Yoshiji Yamada, Motoji Sawabe, Kimihiko Kato, Tamotsu Nishida |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Candidate gene Microarray In Vitro Techniques Protein Serine-Threonine Kinases Biology Epigenesis Genetic FYN Genetics Humans Epigenetics Promoter Regions Genetic Gene Oligonucleotide Array Sequence Analysis Homeodomain Proteins Intracellular Signaling Peptides and Proteins Zinc Finger E-box-Binding Homeobox 1 Promoter General Medicine DNA Methylation Atherosclerosis Molecular biology CpG site DNA methylation Trans-Activators CpG Islands Female Transcription Factors |
| Zdroj: | International Journal of Molecular Medicine. 33:1355-1363 |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2014.1692 |
| Popis: | Epigenetic modification, particularly changes in DNA methylation at gene promoters, is implicated in the pathogenesis of atherosclerosis. However, the analysis of DNA methylation in atherosclerosis has been limited to a few selected candidate genes. In this study, we therefore performed a genome-wide analysis of DNA methylation in the atherosclerotic human aorta. A total of 48 post-mortem human aortic intima specimens were examined. To avoid the effects of interindividual variation, we performed intraindividual paired comparisons between atheromatous plaque lesions and corresponding plaque-free tissue for 24 subjects. Bisulfite-modified genomic DNA was analyzed for DNA methylation with a specific microarray (Illumina HumanMethylation450 BeadChip). We compensated for multiple comparisons by applying Bonferroni's correction for statistical significance of association. DNA methylation was significantly (P |
| Databáze: | OpenAIRE |
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