Bone morphogenetic protein-2 delivery from polyelectrolyte multilayers enhances osteogenic activity on nanostructured titania
| Autor: | Tara Wigmosta, Matt J. Kipper, Ketul C. Popat |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
medicine.medical_treatment 0206 medical engineering Biomedical Engineering Bone Morphogenetic Protein 2 Biocompatible Materials 02 engineering and technology Bone healing Bone morphogenetic protein 2 Osseointegration Biomaterials Chitosan chemistry.chemical_compound Osteogenesis medicine Animals Cells Cultured Titanium Drug Carriers biology Growth factor Metals and Alloys Mesenchymal Stem Cells 021001 nanoscience & nanotechnology 020601 biomedical engineering Polyelectrolyte Nanostructures Rats chemistry Ceramics and Composites Osteocalcin biology.protein Alkaline phosphatase 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of biomedical materials research. Part AREFERENCES. 109(7) |
| ISSN: | 1552-4965 |
| Popis: | Incomplete osseointegration is primary cause of failure for orthopedic implants. New biomaterials that present stable signals promoting osteogenesis could reduce failure rates of orthopedic implants. In this study bone morphogenetic protein-2 (BMP-2) was delivered from titania nanotubes (Nt) modified with chitosan/heparin polyelectrolyte multilayers (PEMs). The surfaces were characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. BMP-2 release from the surfaces was measured in vitro for up to 28 days. After an initial burst release of BMP-2 during the first 2 days, most of the BMP-2 remained on the surface. To determine the osteogenic properties of these surfaces, they were seeded with rat bone marrow cells; alkaline phosphatase (ALP) activity, total protein, calcium deposition, and osteocalcin were measured up to 4 weeks in vitro. When compared to Nt surfaces, the surfaces with BMP-2 induce greater osteocalcin and calcium deposition. PEMs provide sustained presentation of BMP-2, from a biomimetic surface. This enhances the osteogenic properties of the surface without requiring supraphysiologic growth factor dose. This growth factor delivery strategy could be used to improve bone healing outcomes and reduce complications for recipients of orthopedic implants. |
| Databáze: | OpenAIRE |
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