Anti-inflammatory and bronchodilatory constituents of leaf extracts of Anacardium occidentale L. in animal models
| Autor: | Abdullahi A. Adejare, Adedoyin Igunnu, Abiodun Humphrey Adebayo, Bamidele Victor Owoyele, Olubunmi Atolani, Oluwakemi Josephine Awakan, S. O. Malomo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Oleamide medicine.drug_class Bronchoconstriction Guinea Pigs Anti-Inflammatory Agents Inflammation Oleic Acids Pharmacology Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography Edema medicine Animals Humans Anacardium Rats Wistar biology Plant Extracts General Medicine biology.organism_classification Bronchodilator Agents Rats Plant Leaves 030104 developmental biology chemistry Female medicine.symptom 030217 neurology & neurosurgery Histamine |
| Zdroj: | Journal of integrative medicine. 16(1) |
| ISSN: | 2095-4964 |
| Popis: | Anacardium occidentale L. leaf is useful in the treatment of inflammation and asthma, but the bioactive constituents responsible for these activities have not been characterized. Therefore, this study was aimed at identifying the bioactive constituent(s) of A. occidentale ethanolic leaf extract (AOEL) and its solvent-soluble portions, and evaluating their effects on histamine-induced paw edema and bronchoconstriction.The bronchodilatory effect was determined by measuring the percentage protection provided by plant extracts in the histamine-induced bronchoconstriction model in guinea pigs. The anti-inflammatory effect of the extracts on histamine-induced paw edema in rats was determined by measuring the increase in paw diameter, after which the percent edema inhibition was calculated. The extracts were analyzed using gas chromatography-mass spectrometry to identify the bioactive constituents. Column chromatography and Fourier transform infrared spectroscopy were used respectively to isolate and characterize the constituents. The bronchodilatory and anti-inflammatory activities of the isolated bioactive constituent were evaluated.Histamine induced bronchoconstriction in the guinea pigs and edema in the rat paw. AOEL, hexane-soluble portion of AOEL, ethyl acetate-soluble portion of AOEL, and chloroform-soluble portion of AOEL significantly increased bronchodilatory and anti-inflammatory activities (P 0.05). Oleamide (9-octadecenamide) was identified as the most abundant compound in the extracts and was isolated. Oleamide significantly increased bronchodilatory and anti-inflammatory activities by 32.97% and 98.41%, respectively (P 0.05).These results indicate that oleamide is one of the bioactive constituents responsible for the bronchodilatory and anti-inflammatory activity of A. occidentale leaf, and can therefore be employed in the management of bronchoconstriction and inflammation. |
| Databáze: | OpenAIRE |
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