Analysis of Parent Synthetic Cannabinoids in Blood and Urinary Metabolites by Liquid Chromatography Tandem Mass Spectrometry
| Autor: | Justin M. Holler, Barry Levine, Thomas Z. Bosy, Jeffrey D. Chmiel, Gerardo Ramos, Jessica L. Knittel, Joseph Magluilo, Shawn P. Vorce |
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| Rok vydání: | 2016 |
| Předmět: |
Drug
medicine.drug_class Health Toxicology and Mutagenesis media_common.quotation_subject medicine.medical_treatment Urine Toxicology Tandem mass spectrometry 01 natural sciences Designer Drugs Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Synthetic cannabinoids medicine Humans Environmental Chemistry 030216 legal & forensic medicine media_common Chemical Health and Safety Chromatography medicine.diagnostic_test Cannabinoids Chemistry 010401 analytical chemistry Articles 0104 chemical sciences Designer drug Immunoassay Cannabinoid Metabolic Networks and Pathways Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Analytical Toxicology. 40:173-186 |
| ISSN: | 1945-2403 0146-4760 |
| Popis: | Synthetic cannabinoids emerged on the designer drug market in recent years due to their ability to produce cannabis-like effects without the risk of detection by traditional drug testing techniques such as immunoassay and gas chromatography-mass spectrometry. As government agencies work to schedule existing synthetic cannabinoids, new, unregulated and structurally diverse compounds continue to be developed and sold. Synthetic cannabinoids undergo extensive metabolic conversion. Consequently, both blood and urine specimens may play an important role in the forensic analysis of synthetic cannabinoids. It has been observed that structurally similar synthetic cannabinoids follow common metabolic pathways, which often produce metabolites with similar metabolic transformations. Presented are two validated quantitative methods for extracting and identifying 15 parent synthetic cannabinoids in blood, 17 synthetic cannabinoid metabolites in urine and the qualitative identification of 2 additional parent compounds. The linear range for most synthetic cannabinoid compounds monitored was 0.1-10 ng/mL with the limit of detection between 0.01 and 0.5 ng/mL. Selectivity, specificity, accuracy, precision, recovery and matrix effect were also examined and determined to be acceptable for each compound. The validated methods were used to analyze a compilation of synthetic cannabinoid investigative cases where both blood and urine specimens were submitted. The study suggests a strong correlation between the metabolites detected in urine and the parent compounds found in blood. |
| Databáze: | OpenAIRE |
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