Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination
Autor: | Christian Le Goff, Amélie Chadeyras, David B. Wallace, Hafsa Madani, Eeva S.M. Tuppurainen, Salah Hammami, Charles Euloge Lamien, Elexpeter Aba-Adulugba, Geneviève Libeau, Adama Diallo, Philippe Caufour, Velý Gulyaz, Tajelser Adam, Emmanuel Albina, Emna Fakhfakh, Roland Silber |
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Přispěvatelé: | Inconnu, Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA) |
Rok vydání: | 2009 |
Předmět: |
[SDV]Life Sciences [q-bio]
viruses Sequence Homology Poxviridae Infections medicine.disease_cause L73 - Maladies des animaux Capripoxvirus Receptors G-Protein-Coupled 0403 veterinary science Cluster Analysis Caprin ComputingMilieux_MISCELLANEOUS Genetics 0303 health sciences biology Goats 04 agricultural and veterinary sciences Lumpy skin disease virus 3. Good health Receptors Chemokine Ovin Genotype 040301 veterinary sciences Molecular Sequence Data Virus 03 medical and health sciences Viral Proteins Lumpy skin disease Virology Sheeppox virus medicine Animals Poxviridae 030304 developmental biology Sheeppox Bovin Polymorphism Genetic Sheep Goatpox virus Sequence Analysis DNA biology.organism_classification medicine.disease DNA Viral Cattle |
Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 2009, 90, pp.1967-1977. ⟨10.1099/vir.0.010686-0⟩ |
ISSN: | 0022-1317 1465-2099 |
Popis: | The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus–host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 5′ terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic α-helix and a shorter serine-rich C-tail. |
Databáze: | OpenAIRE |
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