Assays for adjuvanticity of new formulations and of carrier proteins for inducing antibody responses to selected immunogens in the squirrel monkeySaimiri sciureus
| Autor: | Bernard Bonnemains, Philippe Chouteau, Olivier Garraud, Eliane Bourreau, Ronald Perraut |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
0301 basic medicine
Immunogen Recombinant Fusion Proteins medicine.medical_treatment Plasmodium falciparum Immunology Radioimmunoassay Antigens Protozoan Antibodies Viral 03 medical and health sciences 0302 clinical medicine Adjuvants Immunologic Viral Envelope Proteins Antigen Adjuvanticity Tetanus Toxoid medicine Animals Immunology and Allergy Saimiri Heat-Shock Proteins biology Immunogenicity Squirrel monkey Antibodies Monoclonal Saimiri sciureus Cell Biology beta-Galactosidase biology.organism_classification Antibodies Bacterial Virology Circumsporozoite protein 030104 developmental biology Immunoglobulin G Immunization Carrier Proteins Adjuvant 030215 immunology |
| Zdroj: | Immunology and Cell Biology. 72:169-175 |
| ISSN: | 0818-9641 |
| DOI: | 10.1038/icb.1994.25 |
| Popis: | Different ways to improve antibody (Ab) responses following immunizations with selected antigens (TT and HSVgD) were investigated, and thus new adjuvant formulations and carrier molecules in a non-human primate experimental host, the squirrel monkey Saimiri sciureus, were assayed. Both quantitative and qualitative humoral responses were determined by means of radio-immunoassays using monoclonal Ab directed at Saimiri IgG. First, the adjuvanticity of the Syntex (SAF-1) adjuvant and of five new adjuvant formulations were assessed towards the selected Ag. This indicated that all the adjuvants induced similar antigen-specific Ab responses, although the adjuvants could modify to some extent the pattern of the qualitative Ab response. Second, we evaluated an adjuvant-free vaccine approach using a synthetic Ag from the circumsporozoite protein of Plasmodium falciparum as immunogen, this Ag being coupled to purified protein derivative (PPD) or to a recombinant heat shock protein from Mycobacterium tuberculosis. These constructs led to good antibody responses as well as an excellent memory effect. Bacille Calmette-Guérin (BCG) priming was required in conjunction with PPD as a carrier molecule to allow homogeneous Ab responses, whereas the heat shock protein construct gave a less homogeneous Ab response regardless of whether a BCG priming was done. We, in addition, discuss the relevance of Saimiri monkeys as experimental models for studies directed at evaluating the immunogenicity of further vaccine candidates. |
| Databáze: | OpenAIRE |
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