Controlled division of cell-sized vesicles by low densities of membrane-bound proteins
| Autor: | Ziliang Zhao, Solveig Mareike Bartelt, Rumiana Dimova, Reinhard Lipowsky, Jan Steinkühler, Roland L. Knorr, Tripta Bhatia, Seraphine V. Wegner |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Low protein Cell division Science General Physics and Astronomy 02 engineering and technology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Membrane biophysics Membrane fission Biophysical chemistry Humans lcsh:Science Synthetic biology Multidisciplinary Artificial cell Chemistry Vesicle Cell Membrane Cytoplasmic Vesicles Membrane Proteins General Chemistry 021001 nanoscience & nanotechnology 030104 developmental biology Membrane Membrane curvature Biophysics lcsh:Q 0210 nano-technology Biological physics Cell Division |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) |
| Popis: | The proliferation of life on earth is based on the ability of single cells to divide into two daughter cells. During cell division, the plasma membrane undergoes a series of morphological transformations which ultimately lead to membrane fission. Here, we show that analogous remodeling processes can be induced by low densities of proteins bound to the membranes of cell-sized lipid vesicles. Using His-tagged fluorescent proteins, we are able to precisely control the spontaneous curvature of the vesicle membranes. By fine-tuning this curvature, we obtain dumbbell-shaped vesicles with closed membrane necks as well as neck fission and complete vesicle division. Our results demonstrate that the spontaneous curvature generates constriction forces around the membrane necks and that these forces can easily cover the force range found in vivo. Our approach involves only one species of membrane-bound proteins at low densities, thereby providing a simple and extendible module for bottom-up synthetic biology. Membrane fission of a cell into two daughters is a core ability of cell-based life. Here the authors show that in artificial cells division can be controlled by regulating membrane curvature using low protein density. |
| Databáze: | OpenAIRE |
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