MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay
| Autor: | Paola Ceruti, Metello Leiss de Leimburg, Elio Berutti, Mario Lendini, Alessandra Angeretti, Damiano Pasqualini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Mineral trioxide aggregate
DNA Bacterial Materials science Aluminum Compounds Bacterial Typing Techniques Calcium Compounds DNA Bacterial Ribosomal Dental Leakage Drug Combinations Enterococcus faecalis Humans Immunoblotting Oxides Polymerase Chain Reaction Root Canal Filling Materials Root Canal Obturation Silicates Tooth Apex Tooth Nonvital Bacterial leakage Dentistry DNA Ribosomal law.invention law Apical foramen General Dentistry Polymerase chain reaction Reverse dot blot Tooth Nonvital biology business.industry Significant difference biology.organism_classification business |
| Zdroj: | Scopus-Elsevier |
| Popis: | Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde apical obturation with MTA; three groups had 1-, 2-, or 3-mm thickness. Sterilized specimens were inoculated with E. faecalis and incubated in sterile medium. DNA extracted from the specimens was amplified by polymerase chain reaction, which yielded a specific segment of E. faecalis 16S rDNA. On day 10 of incubation, no specimens were contaminated. On day 50, almost 17% of specimens were contaminated, with no statistically significant difference between groups (Chi-square = 0.48; df = 2; p = 0.787). Therefore, MTA provides an adequate seal even in cases of orthograde apical obturation of pulpless teeth with open apices. |
| Databáze: | OpenAIRE |
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