Phytogenic silver, gold, and bimetallic nanoparticles as novel antitubercular agents
| Autor: | Manisha Arkile, Laxman Nawale, Utkarsha U. Shedbalkar, Snehal Chopade, Dhiman Sarkar, Sweety A. Wadhwani, Balu A. Chopade, Richa Singh |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plumbago zeylanica Materials science Silver medicine.drug_class mycobacteria 030106 microbiology Biophysics Antitubercular Agents Drug Evaluation Preclinical Pharmaceutical Science Metal Nanoparticles Bioengineering 02 engineering and technology Microbial Sensitivity Tests Pharmacology Antimycobacterial Cell Line Biomaterials Mycobacterium tuberculosis 03 medical and health sciences Minimum inhibitory concentration Plumbaginaceae International Journal of Nanomedicine Acanthaceae Drug Discovery medicine antimycobacterial agent Humans Cytotoxicity Original Research drug resistance Plants Medicinal biology Macrophages Organic Chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Mycobacterium bovis In vitro tuberculosis cytotoxicity nanoparticles Gold 0210 nano-technology Ex vivo Barleria prionitis |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Richa Singh,1 Laxman Nawale,2 Manisha Arkile,2 Sweety Wadhwani,1 Utkarsha Shedbalkar,1 Snehal Chopade,1 Dhiman Sarkar,2 Balu Ananda Chopade1,3 1Department of Microbiology, Savitribai Phule Pune University, 2Combichem-Bioresource Center, Organic Chemistry Division, National Chemical Laboratory, Pune, 3Dr Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India Purpose: Multi- and extensively drug-resistant tuberculosis (TB) is a global threat to human health. It requires immediate action to seek new antitubercular compounds and devise alternate strategies. Nanomaterials, in the present scenario, have opened new avenues in medicine, diagnosis, and therapeutics. In view of this, the current study aims to determine the efficacy of phytogenic metal nanoparticles to inhibit mycobacteria. Methods: Silver (AgNPs), gold (AuNPs), and gold–silver bimetallic (Au–AgNPs) nanoparticles synthesized from medicinal plants, such as Barleria prionitis, Plumbago zeylanica, and Syzygium cumini, were tested against Mycobacterium tuberculosis and M. bovis BCG. In vitro and ex vivo macrophage infection model assays were designed to determine minimum inhibitory concentration (MIC) and half maximal inhibitory concentration of nanoparticles. Microscopic analyses were carried out to demonstrate intracellular uptake of nanoparticles in macrophages. Besides this, biocompatibility, specificity, and selectivity of nanoparticles were also established with respect to human cell lines. Results: Au–AgNPs exhibited highest antitubercular activity, with MIC of |
| Databáze: | OpenAIRE |
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