Alteration of the mare's immune system by the synthetic progestin, altrenogest
| Autor: | Kristina G Lu, Jessica M Hanneman, C. J. Corbin, Amanda A. Adams, C.E. Fedorka, Mats H.T. Troedsson, Olivia F. Walker, Alan J Conley, Barry A. Ball |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Altrenogest peripheral blood mononuclear cell medicine.medical_treatment animal diseases Interleukin-1beta altrenogest Endometrium chemistry.chemical_compound 0302 clinical medicine Glucocorticoid Pregnancy Receptors Leukocytes cytokine glucocorticoid receptor Immunology and Allergy Medicine Progesterone reproductive and urinary physiology media_common equine 030219 obstetrics & reproductive medicine Obstetrics and Gynecology Interleukin-10 medicine.anatomical_structure Cytokine Female Trenbolone Acetate Ovulation medicine.drug_class media_common.quotation_subject Mononuclear Clinical Sciences Immunology progesterone Peripheral blood mononuclear cell Andrology Paediatrics and Reproductive Medicine 03 medical and health sciences Interferon-gamma Receptors Glucocorticoid Immune system Animals Horses Obstetrics & Reproductive Medicine business.industry urogenital system Interleukin-6 Animal Horse 030104 developmental biology Reproductive Medicine chemistry Leukocytes Mononuclear Pregnancy Animal Interleukin-4 business Progestin |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989), vol 82, iss 2 |
| Popis: | Author(s): Fedorka, Carleigh Elizabeth; Ball, Barry A; Walker, Olivia F; Conley, Alan J; Corbin, Cynthia J; Lu, Kristina G; Hanneman, Jessica M; Troedsson, Mats HT; Adams, Amanda A | Abstract: ProblemProgestins are immunomodulatory in a variety of species. In the horse, the most commonly administered synthetic progestin is altrenogest (ALT), but its effect on the immune system of the non-pregnant mare is unknown.MethodsPeripheral blood mononuclear cells (PBMCs) from diestrous mares were incubated with varying concentrations of progesterone (P4) or ALT to assess intracellular production of IFNγ and the expression of select cytokines. Additionally, ten mares received either ALT or VEH daily utilizing a switchback design beginning on the day of ovulation and continuing for 7ndays. Circulating PBMCs and endometrial biopsies were obtained to assess the production and expression of the same cytokines.ResultsIn vitro, both P4 and ALT caused a dose-dependent decrease in intracellular IFNγ in PBMCs. P4 caused a dose-dependent decrease in the expression of IFNγ, IL-10 and IL-4, while ALT caused an increase in the expression of IL-6 and IL-1β in PBMCs. In vivo, ALT suppressed the intracellular levels of IFNγ in PBMCs on d6. While control mares experienced a decrease in IL-1β expression from d0 to d6, ALT-treated mares did not. In the endometrium, ALT increased the expression of IL-1RN and IFNγ in comparison with VEH-treated mares.ConclusionP4 and ALT appear to alter the immune system of the non-pregnant mare both systemically in addition to locally within the endometrium. Further research is necessary to determine the pathways through which this synthetic progestin functions on the immune system of the horse, and the consequences it may have. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|