Protein engineering of nitrilase for chemoenzymatic production of glycolic acid
| Autor: | Shijun Wu, Raymond E. Jackson, Mark S. Payne, Arie Ben-Bassat, Robert DiCosimo, Kimberley N. Parker, Kelly L. Petrillo, Arthur J. Fogiel, Daniel P. O'Keefe |
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| Rok vydání: | 2007 |
| Předmět: |
Glycolonitrile
Acetonitriles biology Chemistry Acidovorax facilis Betaproteobacteria Bioengineering Protein engineering biology.organism_classification Protein Engineering Applied Microbiology and Biotechnology Nitrilase Recombinant Proteins Enzyme catalysis Glycolates chemistry.chemical_compound Biochemistry Aminohydrolases Escherichia coli Fermentation Ammonium Glycolic acid Biotechnology |
| Zdroj: | Biotechnology and bioengineering. 99(3) |
| ISSN: | 1097-0290 |
| Popis: | A key step in a chemoenzymatic process for the production of high-purity glycolic acid (GLA) is the enzymatic conversion of glycolonitrile (GLN) to ammonium glycolate using a nitrilase derived from Acidovorax facilis 72W. Protein engineering and over-expression of this nitrilase, combined with optimized fermentation of an E. coli transformant were used to increase the enzyme-specific activity up to 15-fold and the biocatalyst-specific activity up to 125-fold. These improvements enabled achievement of the desired volumetric productivity and biocatalyst productivity for the conversion of GLN to ammonium glycolate. |
| Databáze: | OpenAIRE |
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