Characterization of insulin antibodies by Surface Plasmon Resonance in two clinical cases: Brittle Diabetes and Insulin Autoimmune Syndrome

Autor: Natalia Ines Faccinetti, Silvina Noemi Valdez, Luciano Lucas Guerra, María C. Arriazu, Edgardo Poskus, Aldana Trabucchi, Andrea G. Krochik, Ruben F. Iacono
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
SURFACE PLASMON RESONANCE
Anatomy and Physiology
medicine.medical_treatment
Insulin Antibodies
lcsh:Medicine
CONCENTRATION AND AFFINITY OF (AUTO)ANTIBODIES
INSULIN (AUTO)ANTIBODIES
Endocrinology
Immune Physiology
Insulin
Surface plasmon resonance
lcsh:Science
Child
Immune Response
Multidisciplinary
biology
food and beverages
purl.org/becyt/ford/3.1 [https]
Medicina Básica
Medicine
Female
purl.org/becyt/ford/3 [https]
Antibody
Research Article
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Adolescent
Inmunología
Immunoglobulins
Hypoglycemia
Antibodies
Autoimmune Diseases
Immune system
HYPO AND HYPERGLYCEMIA
Internal medicine
Diabetes mellitus
medicine
Humans
Immunoassays
Aged
Autoantibodies
Diabetic Endocrinology
Type 1 diabetes
business.industry
lcsh:R
Autoantibody
Infant
Diabetes Mellitus Type 1
Surface Plasmon Resonance
medicine.disease
Diabetes Mellitus
Type 1
biology.protein
Immunologic Techniques
lcsh:Q
Clinical Immunology
business
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE
PLoS ONE, Vol 8, Iss 12, p e84099 (2013)
DOI: 10.1371/journal.pone.0084099
Popis: In this study, the characterization of insulin (auto)antibodies has been described, mainly in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by Surface Plasmon Resonance (SPR) in two particular clinical cases of individuals with severe episodes of impaired glycemia. Subject 1 suffers from brittle diabetes associated with circulating insulin antibodies (IA) due to insulin treatment. Subject 2 has insulin autoantibodies (IAA) associated with hypoglycemia in spite of not being diabetic and not having ever received exogenous insulin therapy. After conventional screening for IA/IAA by radioligand binding assay (RBA), we further characterized IA/IAA in sera of both patients in terms of concentration (q), affinity (Ka) and Ig (sub)isotypes by means of SPR technology. In both cases, q values were higher and Ka values were lower than those obtained in type 1 diabetic patients, suggesting that IA/IAA:insulin immunocomplexes could be responsible for the uncontrolled glycemia. Moreover, subject 1 had a predominat IgG1 response and subject 2 had an IgG3 response. In conclusion, SPR technology is useful for the complete characterization of IA/IAA which can be used in special cases where the simple positive/negative determination is not enough to achieve a detailed description of the disease fisiopathology. Fil: Trabucchi, Aldana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina Fil: Iacono, Ruben Francisco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina Fil: Guerra, Luciano Lucas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina Fil: Faccinetti, Natalia Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina Fil: Krochik, Andrea G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina Fil: Arriazu, María C.. Hospital Comunitario Privado; Argentina Fil: Poskus, Edgardo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Valdez, Silvina Noemi. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Databáze: OpenAIRE
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