Combined lead and zinc oxide-nanoparticles induced thyroid toxicity through 8-OHdG oxidative stress-mediated inflammation, apoptosis, and Nrf2 activation in rats
| Autor: | Eman El-Sayed Khayal, Arwa A. El-Sheikh, Amany Mohamed Shalaby, Hanaa M. Ibrahim, Mohamed Ali Alabiad |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty NF-E2-Related Factor 2 Health Toxicology and Mutagenesis Thyroid Gland Apoptosis Management Monitoring Policy and Law Toxicology medicine.disease_cause chemistry.chemical_compound Internal medicine medicine Animals Inflammation Triiodothyronine Chemistry Thyroid General Medicine Malondialdehyde Rats Oxidative Stress medicine.anatomical_structure Endocrinology Lead Lead acetate 8-Hydroxy-2'-Deoxyguanosine Toxicity Nanoparticles Zinc Oxide Oxidative stress Hormone |
| Zdroj: | Environmental toxicologyREFERENCES. 36(12) |
| ISSN: | 1522-7278 |
| Popis: | A human is exposed to a chemical mixture rather than a single chemical, particularly with the wide spread of nanomaterials. Therefore, the present study evaluated the combined exposure of lead acetate (Pb) and zinc oxide-nanoparticles (ZnO-NPs) compared to each metal alone on the thyroid gland of adult rats. A total of 30 adult male albino rats were divided into four groups, group I (control), group II received Pb (10 mg/kg), group III received ZnO-NPs (85 mg/kg) and group IV co-administrated the two metals in the same previous doses. The materials were gavaged for 8 weeks. The toxicity was assessed through several biochemical parameters. Our results revealed significant body weight reduction relative to increased thyroid weights, decreased both of serum-free triiodothyronine (FT3), tetra-iodothyronine (FT4), increased thyroid-stimulating hormone (TSH), increased serum and thyroid levels of Pb and zinc, significant elevation in tumor necrosis factor-α (TNF-α), reduction in interleukin 4 (IL4), upregulation of Bax, and downregulation of Bcl-2 genes. Additionally, there was significant overexpression of nuclear factor erythroid 2-related factor 2(Nrf2), 8-Hydroxydeoxyguanosine(8-OHdG), the elevation of tissues malondialdehyde (MDA), reduction of tissues total antioxidant capacity (TAC), and disruptive thyroid structural alterations in all metals groups with marked changes in the combined metals group. In conclusion, the combined exposure of Pb and ZnO-NPs induced pronounced toxic thyroid injury, pointing to additive effects in rats than the individual metal effects through different significant changes of disruptive thyroid structural alterations related to the loading of thyroid tissues with Pb and zinc metals producing oxidative stress that mediated inflammation and apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text