α-Synuclein overexpression during manganese-induced apoptosis in SH-SY5Y neuroblastoma cells
| Autor: | Tongjian Cai, Liping Ye, Ying Zhang, Liguang Sun, Yu Wang, Shijie Lv, Yan Li |
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| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Transcription Genetic Blotting Western Apoptosis Biology Flow cytometry Chlorides Transcription (biology) Cell Line Tumor Neuroblastoma medicine Humans RNA Messenger Annexin A5 medicine.diagnostic_test Caspase 3 Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Neurotoxicity medicine.disease Molecular biology Trace Elements nervous system diseases Blot Manganese Compounds nervous system Cell culture Protein Biosynthesis alpha-Synuclein Intracellular |
| Zdroj: | Brain Research Bulletin. 81:428-433 |
| ISSN: | 0361-9230 |
| Popis: | Chronic inorganic manganese (Mn) exposure has been known to induce neurological disorders similar to Parkinson's disease (PD). Apoptosis has been shown to be involved in manganese-induced neurotoxicity. However, the up-stream molecular mechanisms for cell apoptosis are not established. alpha-Synuclein (alpha-syn) is a major component of intracellular inclusions in PD, Alzheimer's disease (AD), and other neurodegenerative disorders. We investigated the role of alpha-syn in manganese chloride (MnCl(2))-induced apoptosis. Results show that MnCl(2) enhanced transcriptional and translational alpha-syn overexpression, and apoptosis as measured by caspase-3 activity and flow cytometry. Overexpressing alpha-syn exacerbated manganese-induced apoptosis, whereas antisense alpha-syn treatment significantly reversed MnCl(2)-induced apoptosis in human neuroblastoma SH-SY5Y cells. In conclusion, our results imply that intracellular alpha-syn overexpression may be responsible for MnCl(2)-induced apoptosis. |
| Databáze: | OpenAIRE |
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