Use of a polyclonal antibody for the determination of the prognostic value of c-erbB-2 protein over-expression in human breast cancer
| Autor: | Philippe Terrier, H. Mouriesse, Brigitte Loridon, F. May-Levin, J. C. Delarue, Marianne Gotteland |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Antibodies Neoplasm Receptor ErbB-2 Mammary gland Breast Neoplasms Epitope Epitopes Breast cancer Risk Factors Carcinoma Medicine Animals Humans Radiology Nuclear Medicine and imaging RNA Messenger skin and connective tissue diseases Coloring Agents Paraffin Embedding biology business.industry Cancer Hematology General Medicine Middle Aged medicine.disease Prognosis Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Polyclonal antibodies Multivariate Analysis Cancer research biology.protein Linear Models Female Rabbits Antibody Neoplasm Recurrence Local business |
| Zdroj: | Acta oncologica (Stockholm, Sweden). 35(1) |
| ISSN: | 0284-186X |
| Popis: | A rabbit-specific polyclonal antibody was obtained raised to a synthetic peptide corresponding to the 1238-1255 C-terminal predicted sequence of the c-erbB-2 protein. This antibody was used in an immunohistochemical procedure to detect the c-erbB-2 protein on a series of 88 paraffin-embedded human breast carcinomas. In 14/88 cases (16%) the c-erbB-2 protein was found to be overexpressed (immunohistochemical score > 1) with a good concordance with the previously determined mRNA level (79/88 cases: 90%). Prognostic significance of c-erbB-2 protein overexpression as detected by immunohistochemistry was tested by the log-rank test. The relative risk of relapse is higher for patients with an immunohistochemical score > 1 (p = 0.00002). In a multivariate analysis of the c-erbB-2 immunohistochemical score was the only powerful parameter (p < 1 x 10(-3). In conclusion, this antibody seems to be a valuable tool in estimating the c-erbB-2 protein regarded in our series as a parameter able to identify a subgroup of operable breast cancer patients with a high risk of relapse. |
| Databáze: | OpenAIRE |
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