Additional file 2 of Targeted quantitation of CFTR protein expression in vivo using immunoprecipitation & parallel reaction monitoring tandem mass spectrometry

Autor: Wang, Hui, Dai, Yunxiang, Clark, Nicholas, Boeglin, Lianne, Woo, Caroline, Wooster, Richard, Sun, Gang, Sullivan, James C.
Rok vydání: 2022
DOI: 10.6084/m9.figshare.19719911.v1
Popis: Additional file 2: Figure S1. Peptide mapping for trypsin/LysC digests of recombinant human CFTR protein (UniProtKB-P13569). Figure S2. Standard curve of hCFTR peptide LSLVPDSEQGEAILPR peak area ratio vs protein centration in 50 mg mouse lung membrane protein extracts and 4% BSA-PBST buffer. hCFTR concentration was 0.5, 1.4, 4.1, 12, and 111 pg per mg tissue. Figure S3. Human CFTR peptide LSLVPDSEQGEAILPR in wild type human lung tissue after IP-MS Analysis. A and B represent two different human lungs. Figure S4. Antibody screening using targeted quantification of peptide LSLVPDSEQGEAILPR with replicates.
Databáze: OpenAIRE