A Novel In Vitro Tool to Study Cyst Nematode Chemotaxis
| Autor: | Virginie Gobert, Azeddine Driouich, Anne-Claire Le Roux, Isabelle Boulogne, Eric Nguema-Ona, Maxime Gotté, Maïté Vicré, Sylvain Fournet, Mé Lina Ianszen, Carole Plasson, Bruno Ngala, Yohana Laloum |
|---|---|
| Přispěvatelé: | Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Fédération Nationale des Producteurs de Plants de Pomme de Terre (FN3PT), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Mondial d'Innovation - Groupe Roullier (CMI ), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre Mondial de l'Innovation Roullier, Groupe Roullier |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine [SDV.BIO]Life Sciences [q-bio]/Biotechnology Population Potato cyst nematode Plant Science [SDV.BC]Life Sciences [q-bio]/Cellular Biology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] lcsh:Plant culture tomato (Solanum lycopersicum L) 01 natural sciences root exudates law.invention 03 medical and health sciences cyst nematode potato (Solanum tuberosum L) law [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Methods Bioassay [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 Globodera pallida [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] education [SDV.BDD]Life Sciences [q-bio]/Development Biology two-choice in vitro assay education.field_of_study biology Petri dish Chemotaxis [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology potato (Solanum tuberosum L.) biology.organism_classification In vitro [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding 030104 developmental biology Nematode Biochemistry tomato (Solanum lycopersicum L.) root border cells 010606 plant biology & botany attraction |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2020, 11, pp.1024. ⟨10.3389/fpls.2020.01024⟩ Frontiers in Plant Science, Vol 11 (2020) Frontiers in Plant Science, 2020, 11, pp.1024. ⟨10.3389/fpls.2020.01024⟩ |
| ISSN: | 1664-462X |
| Popis: | This study presents a novel three-dimensional (3D) tool "3D in vitro choice" for chemotaxis assays with cyst nematodes. The original 3D in vitro choice was customized through digital printing. Freshly hatched second stage juveniles (J2s) of the cyst nematode Globodera pallida were used as the nematode model to illustrate chemo-orientation behavior in the 3D system. The efficiency and reliability of the 3D in vitro choice were validated with 2% Phytagel as navigation medium, in three biological assays and using tomato root exudates or potato root border cells and their associated mucilage as a positive attractant as compared with water. For each biological assay, J2s were hatched from the same population of a single generation glasshouse-cultured cysts. This novel easy to use and low-cost 3d-device could be a useful replacement to Petri dishes assays in nematode behavioral studies due to the ease of deposition of nematodes and test substances, coupled with its distinctive zones that allow for precision in choice making by the nematodes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|