Comparative studies of two vegetal extracts from Stokesia laevis and Geranium pratense: polyphenol profile, cytotoxic effect and antiproliferative activity
| Autor: | Georgeta Neagu, Iulian Terchescu, Bujor Albu, Amalia Stefaniu, Lucia Pirvu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Stokesia laevis meadow cranesbill Geranium pratense 03 medical and health sciences Condensed Matter::Materials Science 0302 clinical medicine Drug likeness Materials Chemistry Physics::Atomic and Molecular Clusters Cytotoxic T cell Physics::Chemical Physics stokes’ aster QD1-999 Quantitative Biology::Biomolecules Traditional medicine biology Chemistry General Chemistry biology.organism_classification 030104 developmental biology Polyphenol bioactivity 030220 oncology & carcinogenesis Astrophysics::Earth and Planetary Astrophysics cytotoxicity and antiproliferative effects drug-likeness |
| Zdroj: | Open Chemistry, Vol 18, Iss 1, Pp 488-502 (2020) |
| ISSN: | 2391-5420 |
| Popis: | In this study, two ethanolic extracts, from Stokesia aster (Slae26) and Geranium pratense (Gpre36) respectively, were evaluated in order to assess the cytotoxic activity and potential antiproliferative activity upon the nontumorigenic human epithelial cell line derived from the mammary gland (MCF-12A) and the human breast tumor cell line (BT-20). The selection of the plant species was done on the basis of their chemical composition, specifically combinations of luteolin derivatives with caffeic and gallic acid derivatives. Therefore, the S. laevis ethanolic extract proved its capacity to inhibit the viability of both normal and tumor breast cell lines (i.e., up to 90% cell viability inhibition, IC50 = 42 µg/mL). On the contrary, the G. pratense ethanolic extract proved weak stimulatory effects on the viability of the two human breast cell lines studied. The obtained results were discussed in the contexts of computational studies and drug-likeness bioactivity of seven common luteolin derivatives: luteolin, luteolin-7-O-glucoside/cynaroside, luteolin-5-O-glucoside/galuteolin, luteolin-6-C-glucoside/isoorientin, luteolin-8-C-glucoside/orientin, luteolin-3′,4′-di-O-glucoside and luteolin-7,3′-di-O-glucoside. Computational studies have revealed that the hydrophilic behavior of luteolin derivatives (log P values) does not follow other tested parameters (e.g., polar surface area values), possibly explaining different efficacy concerning the biological properties in vitro. These predictions could be a starting point for studies on the biochemical mechanism by which luteolin derivatives induce biological effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|