Chronic myelocytic leukemia: ultrastructural histopathology of bone marrow from patients in the chronic phase
| Autor: | Yumi Horasawa, Hideo Tokuhiro, Yoshihiko Watanabe, Takashi Nagaoka, Yasushi Hirasawa, Masatoshi Hayashi, Fumio Kuto |
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| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Pathology Biopsy Biology Granulopoiesis Pathology and Forensic Medicine Structural Biology Reticular cell Bone Marrow medicine Freeze Fracturing Humans medicine.diagnostic_test Mononuclear phagocyte system Middle Aged medicine.disease Haematopoiesis Leukemia Microscopy Electron medicine.anatomical_structure Leukemia Myeloid Microscopy Electron Scanning Histopathology Female Bone marrow |
| Zdroj: | Ultrastructural pathology. 6(4) |
| ISSN: | 0191-3123 |
| Popis: | We investigated the ultrastructural changes in the hematopoietic microenvironment of the bone marrow obtained from 15 untreated patients with chronic myelocytic leukemia (CML) in the chronic phase by transmission and scanning electron microscopy using the cryofracture technique. Examination of the undecalcified bone marrow specimens confirmed extensive hyperplastic granulopoiesis. In the stroma, fat cells were scarce or absent. Macrophages were increased and scattered throughout the marrow. The cytoplasm contained abundant cellular debris and crystals of the Charcot-Leyden type. Slender reticular cells were inconspicuously located between proliferating myeloid cells. A few foci of fibrosis were occasionally observed. The sinus endothelium generally retained its continuity, and no features suggesting complete deterioration of the sinus were evident. However, certain alterations in the sinus wall were noted in the process of leukemia cell migration. Many cells migrated transcellularly into the circulation through transient large openings in the sinus endothelium. |
| Databáze: | OpenAIRE |
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