Differential Regulation of Inflammatory Cytokine Secretion by Human Dendritic Cells upon Chlamydia trachomatis Infection
| Autor: | Jean François Maisonneuve, Robert J. Suchland, Kenneth H. Grabstein, Ana L. Gervassi, Mark R. Alderson, Peter Probst |
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| Rok vydání: | 2004 |
| Předmět: |
Immunology
Caspase 1 Chlamydia trachomatis Receptors Cell Surface Biology medicine.disease_cause Microbiology Proinflammatory cytokine Immune system medicine Humans Secretion Host Response and Inflammation Membrane Glycoproteins Chlamydia Toll-Like Receptors Interleukin-18 Dendritic Cells Dendritic cell Chlamydia Infections medicine.disease Enzyme Activation Toll-Like Receptor 4 Infectious Diseases Cytokines Parasitology Cytokine secretion Cell Adhesion Molecules Interleukin-1 |
| Zdroj: | Infection and Immunity. 72:7231-7239 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.72.12.7231-7239.2004 |
| Popis: | Chlamydia trachomatis is an obligate intracellular gram-negative bacterium responsible for a wide spectrum of diseases in humans. Both genital and ocular C. trachomatis infections are associated with tissue inflammation and pathology. Dendritic cells (DC) play an important role in both innate and adaptive immune responses to microbial pathogens and are a source of inflammatory cytokines. To determine the potential contribution of DC to the inflammatory process, human DC were infected with C. trachomatis serovar E or L2. Both C. trachomatis serovars were found to infect and replicate in DC. Upon infection, DC up-regulated the expression of costimulatory (B7-1) and cell adhesion (ICAM-1) molecules. Furthermore, chlamydial infection induced the secretion of interleukin-1β (IL-1β), IL-6, IL-8, IL-12p70, IL-18, and tumor necrosis factor alpha (TNF-α). The mechanisms involved in Chlamydia -induced IL-1β and IL-18 secretion differed from those of the other cytokines. Chlamydia -induced IL-1β and IL-18 secretion required infection with viable bacteria and was associated with the Chlamydia -induced activation of caspase-1 in infected host cells. In contrast, TNF-α and IL-6 secretion did not require that the Chlamydia be viable, suggesting that there are at least two mechanisms involved in the Chlamydia -induced cytokine secretion in DC. Interestingly, an antibody to Toll-like receptor 4 inhibited Chlamydia -induced IL-1β, IL-6, and TNF-α secretion. The data herein demonstrate that DC can be infected by human C. trachomatis serovars and that chlamydial components regulate the secretion of various cytokines in DC. Collectively, these data suggest that DC play a role in the inflammatory processes caused by chlamydial infections. |
| Databáze: | OpenAIRE |
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