The Modulation of Cytokine and IgE Production by Tumor Necrosis Factor-β in Atopic Dermatitis
| Autor: | Heather L. Donahue, Donald Y. M. Leung, Anne E. Trumble, Orathai Jirapongsananuruk |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Allergy medicine.drug_class medicine.medical_treatment Dermatology Immunoglobulin E Monoclonal antibody Biochemistry Peripheral blood mononuclear cell Monocytes Dermatitis Atopic Pathogenesis Atopy Interferon-gamma Reference Values Humans Medicine Lymphotoxin-alpha Molecular Biology Cells Cultured Interleukin-13 atopic dermatitis biology business.industry Cell Biology Middle Aged allergy medicine.disease Up-Regulation Cytokine Immunology biology.protein Cytokines Female Tumor necrosis factor alpha IgE TNF-β Interleukin-5 business |
| Zdroj: | Journal of Investigative Dermatology. 114:200-203 |
| ISSN: | 0022-202X |
| DOI: | 10.1046/j.1523-1747.2000.00847.x |
| Popis: | Atopic dermatitis (AD) is associated with increased IL-4, IL-5, and IL-13 but decreased IFN-gamma production. This cytokine profile may account for the atopic features of this illness, including IgE upregulation. Recent studies have demonstrated that tumor necrosis factor (TNF)-beta is produced by Th1-like cells, but the cytokine modulation by TNF-beta and the clinical significance of this cytokine in AD is not known. Therefore, this study was carried out to determine the potential role of TNF-beta in AD. In this study, we cultured peripheral blood mononuclear cells from patients with AD and normal subjects with anti-CD3 monoclonal antibodies and investigated the production of TNF-beta by ELISA. The mean +/- SEM of TNF-beta production in AD was significantly lower than normal subjects (p = 0.03). The effect of TNF-beta on cytokine production was investigated by culturing peripheral blood mononuclear cells with anti-CD3 monoclonal antibodies in the presence or absence of TNF-beta. Compared with medium control, TNF-beta significantly decreased IL-5 (p = 0.0004) and IL-13 (p = 0.008) but increased IFN-gamma (p = 0.001) production. The effect of TNF-beta on IgE production was determined by culturing peripheral blood mononuclear cells in the IL-4- and anti-CD40-induced IgE production system. Interestingly, TNF-beta significantly decreased IgE (p = 0.02), but not IgG production compared with medium control. Our study demonstrates that TNF-beta production is downregulated in AD. This cytokine increases IFN-gamma production but decreases IL-5, IL-13, as well as IgE production. These findings suggest a potential role for TNF-beta in the pathogenesis of AD. |
| Databáze: | OpenAIRE |
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