High-performance liquid chromatography of non-polar retinoid isomers

Autor: Gottfried N. Nöll, Christian Becker
Rok vydání: 2000
Předmět:
Zdroj: Journal of Chromatography A. 881:183-188
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(00)00232-6
Popis: Normal-phase high-performance liquid chromatograms of retinal, retinol and retinyl palmitate isomers using n-heptane-tert.-butyl methyl ether as mobile phase are presented. Methods for the synthesis of various isomers of these retinoids are described. This enables one to produce standard chromatograms and to select various isomers for cochromatography and the identification of the various peaks under study. Assignment of the peaks is based on chromatograms published previously in this journal. For the main isomers it is possible to get baseline separation of the commonly occurring isomeric forms in a reasonably short analysis time.
Databáze: OpenAIRE