Effects of benzo(a)pyrene on the expression of heat shock proteins, pro-inflammatory cytokines and antioxidant enzymes in hepatic tumors induced by rat hepatoma N1-S1 cells
| Autor: | Nam Hee Won, Hyung Sik Kang, Zhi Zheng, Min Lee, Donggeun Sul, Sohee Phark, So-Young Park |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Antioxidant Carcinoma Hepatocellular medicine.medical_treatment medicine.disease_cause Antioxidants Proinflammatory cytokine Superoxide dismutase Rats Sprague-Dawley chemistry.chemical_compound Hsp27 Heat shock protein Cell Line Tumor medicine Benzo(a)pyrene Animals Humans Oncology & Hematology N1-S1 Rat Hepatoma Cells Heat-Shock Proteins biology Chemistry Liver Neoplasms General Medicine Neoplasms Experimental Molecular biology Rats Oxidative Stress Biochemistry biology.protein Cytokines Tumor necrosis factor alpha Original Article Oxidative stress |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1598-6357 |
| Popis: | Benzo(a)pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that is easily introduced to humans via consumption of grilled or smoked meat. BaP causes harmful oxidative effects on cell development, growth and survival through an increase in membrane lipid peroxidation, oxidative DNA damage and mutagenesis. Therefore, the present study was conducted to evaluate the synergistic effects of BaP on oxidative stress in hepatic tumors. In this study, we established a hepatic tumor model by injecting rat hepatoma N1-S1 cells into healthy rats. Changes in the abundance of heat shock proteins (HSPs), antioxidant enzymes and pro-inflammatory cytokines were then investigated by western blot analysis. In addition, we examined changes in oxidative stress levels. Injection of N1-S1 cells or concomitant injection of BaP and N1-S1 cells resulted in the formation of hepatic tumors at the injection site. Evaluation of rat plasma reveals that hepatic tumors induced by BaP and N1-S1 cells expresses higher levels of Hsp27, superoxide dismutase (SOD), and tumor necrosis factor-α (TNF-α) when compared to those induced by N1-S1 cells only. The collective results of this study suggest that BaP exerts synergistic effects on the expression of HSP, cytokines and antioxidant enzymes in hepatic tumors induced by rat hepatoma N1-S1 cells. |
| Databáze: | OpenAIRE |
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