Two-color immunofluorescence using a fluorescence-activated cell sorter
| Autor: | Michael R. Loken, D R Parks, Leonore A. Herzenberg |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Histology
Fluorescent Antibody Technique Cell Separation Immunofluorescence Immunoglobulin D Rhodamine Flow system chemistry.chemical_compound medicine Lymphocytes Fluorescein biology medicine.diagnostic_test Chemistry Rhodamines Fluoresceins Molecular biology eye diseases Fluorescence activated cell sorter Immunoglobulin M biology.protein Biophysics Anatomy Spleen |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 25(7) |
| ISSN: | 0022-1554 |
| Popis: | A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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