MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
| Autor: | Yukun Wang, Ziming Wang, Xin-Shuai Wang, Limin Wei, Xinyang Li, Guangping Zhang, Siyuan Hu, Zhiwei Liu, Dan-Yang Han |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Bioinformatics miR-16-5p Proliferation Gene Expression ANLN Apoptosis Breast Neoplasms Biology Flow cytometry Breast cancer Cell Movement Cell Line Tumor Gene expression microRNA Databases Genetic Genetics medicine Humans Neoplasm Invasiveness Breast RC254-282 Cell Proliferation Reporter gene medicine.diagnostic_test Cell growth Microfilament Proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens Cell cycle Prognosis Up-Regulation G2 Phase Cell Cycle Checkpoints MicroRNAs Oncology Cancer research M Phase Cell Cycle Checkpoints Female Research Article |
| Zdroj: | BMC Cancer BMC Cancer, Vol 21, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1471-2407 |
| Popis: | Background In recent years, gene expression-based analysis has been used for disease biomarker discovery, providing ways for better diagnosis, leading to improvement of clinical treatment efficacy. This study aimed to explore the role of miR-16-5p and ANLN in breast cancer (BC). Methods Cohort datasets of BC were obtained from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) and analyzed by bioinformatics tools. qRT-PCR and western blotting were applied to validate ANLN and its protein expression. A dual-luciferase reporter assay was used to prove the regulatory relationship of miR-16-5p and ANLN. Finally, MTT, wound healing, Transwell invasion and flow cytometry analyses of the cell cycle and apoptosis were performed to assess cell proliferation, migration, invasion, cell cycle and apoptosis, respectively. Results A total of 195 differentially expressed genes (DEGs) and 50 overlapping microRNAs (miRNAs) were identified. Among these DEGs and miRNAs, ANLN, associated with poor overall survival in BC, overlapped in the GSE29431, GSE42568, TCGA and GEPIA2 databases. Moreover, ANLN was highly expressed, while miR-16-5p was lower in BC cells than in breast epithelial cells. Then, we confirmed that ANLN was directly targeted by miR-16-5p in BC cells. Over-expression of miR-16-5p and knock-down of ANLN remarkably inhibited cell proliferation and migration as well as cell invasion, arrested the cells in G2/M phase and induced apoptosis in BC cells. Conclusions These findings suggest that miR-16-5p restrains proliferation, migration and invasion while affecting cell cycle and promotes apoptosis by regulating ANLN, thereby providing novel candidate biomarkers for the diagnosis and treatment of BC. |
| Databáze: | OpenAIRE |
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