The different anion transport capability of prodiginine- and tambjamine-like molecules
| Autor: | Oscar Moran, Michele Fiore, María García-Valverde, Israel Carreira-Barral |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Iodide Anion Transport Proteins Halide Chloride Gluconates 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Molecule Animals Pyrroles Anion binding Pharmacology chemistry.chemical_classification Ion Transport Chemistry Prodigiosin Combinatorial chemistry Rats Inbred F344 Rats Dissociation constant 030104 developmental biology Membrane Tambjamine 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | European journal of pharmacology. 889 |
| ISSN: | 1879-0712 |
| Popis: | Prodiginines and tambjamines are anion-selective ionophores capable of facilitating the transport of anions across the plasma membrane in mammalian cells. One of the potential applications of these anionophores is the possibility of employing them as a substitutive therapy for pathologies involving anion channels, as in cystic fibrosis. We have studied the interaction of a large anion as gluconate with three prodiginine- and two tambjamine-like compounds. Apparent dissociation constants for the chloride, iodide and gluconate complexes were estimated from iodide influx experiments in mammalian cells exposed to different extracellular anion combinations. Our experiments indicate that gluconate is not transported by the prodiginines, leaving the anionophores free to transport chloride and iodide. Conversely, gluconate would be transported to some extent by the tambjamines, competing with halides for the anionophores, and consequently reducing their flux. This might be related to the different structural features of both families of compounds. These data have important implications for the selection of impermeable anions in the analysis of the anionophore mechanism. |
| Databáze: | OpenAIRE |
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