Study on the relationship between methylation status of HPV 16 E2 binding sites and cervical lesions
| Autor: | Xiaofu Li, Huanhuan Li, Ya Li, Peipei Du, Yanfang Zhi, Guomei Cheng, Tingting Fan, Xiaoan Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Pathology medicine.medical_specialty Clinical Biochemistry Uterine Cervical Neoplasms Biochemistry Asymptomatic Methylation 03 medical and health sciences 0302 clinical medicine medicine BDNA test Humans Prospective Studies RNA Messenger Binding site Aged Cervical cancer Human papillomavirus 16 Binding Sites business.industry Biochemistry (medical) Papillomavirus Infections RNA General Medicine Middle Aged medicine.disease Uterine Cervical Dysplasia 030104 developmental biology Mrna level 030220 oncology & carcinogenesis Pyrosequencing Female medicine.symptom business |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 493 |
| ISSN: | 1873-3492 |
| Popis: | The aim of this study was to investigate the methylation status of E2BSs in the HPV 16 long control region (LCR) in clinical cervical samples.Methylation status of the four E2BSs in 43 clinical cervical samples with HPV 16 infection was quantitatively detected using pyrosequencing. Meanwhile, Quantivirus® HPV E6/E7 RNA 3.0 assay (bDNA) was used to detect E6/E7 mRNA levels in the corresponding specimens.Our results showed that methylation status of E2BS1, 2 and 4 sites in high-grade squamous intraepithelial lesions (HSIL) and cervical cancer were significantly higher than that of asymptomatic HPV 16 infection and low-grade squamous intraepithelial lesions (LSIL) (all P .05). Furthermore, methylation status of HPV 16 E2BS1 and 2 was positively correlated with E6/E7 mRNA levels (rThe methylation status of E2BS1 and 2 may have utility as diagnostic markers for the severity of cervical lesions in the future. |
| Databáze: | OpenAIRE |
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