Characterization and anti-biofilm activity of extracellular polymeric substances produced by the marine biofilm-forming bacterium Pseudoalteromonas ulvae strain TC14
| Autor: | Annick Ortalo-Magné, Gérald Culioli, Linda Guentas-Dombrowsky, Yves Blache, Ahmad Fahs, Maëlle Molmeret, Florence Brian-Jaisson, Stéphane Cérantola |
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| Přispěvatelé: | Laboratoire Insulaire du Vivant et de l'Environnement (LIVE), Université de la Nouvelle-Calédonie (UNC) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] 030106 microbiology Aquatic Science Microscopy Atomic Force Polysaccharide Applied Microbiology and Biotechnology Biofouling 03 medical and health sciences Extracellular polymeric substance Pseudoalteromonas Marine bacteriophage 14. Life underwater Water Science and Technology Glucan chemistry.chemical_classification biology Polysaccharides Bacterial Biofilm biology.organism_classification 030104 developmental biology Biochemistry chemistry Biofilms Water Microbiology Bacteria |
| Zdroj: | Biofouling Biofouling, 2016, Vol.32(5), ⟨10.1080/08927014.2016.1164845⟩ |
| DOI: | 10.1080/08927014.2016.1164845⟩ |
| Popis: | This study investigated soluble (Sol-EPS), loosely bound (LB-EPS), and tightly bound extracellular polymeric substances (TB-EPS) harvested from biofilm and planktonic cultures of the marine bacterium Pseudoalteromonas ulvae TC14. The aim of the characterization (colorimetric methods, FTIR, GC-MS, NMR, HPGPC, and AFM analyses) was to identify new anti-biofilm compounds; activity was assessed using the BioFilm Ring Test®. A step-wise separation of EPS was designed, based on differences in water-solubility and acidity. An acidic fraction was isolated from TB-EPS, which strongly inhibited biofilm formation by marine bacterial strains in a concentration-dependent manner. The main constituents of this fraction were characterized as two glucan-like polysaccharides. An active poly(glutamyl-glutamate) fraction was also recovered from TB-EPS. The distribution of these key EPS components in Sol-EPS, LB-EPS, and TB-EPS was distinct and differed quantitatively in biofilm vs planktonic cultures. The anti-biofilm potential of the fractions emphasizes the putative antifouling role of EPS in the environment. |
| Databáze: | OpenAIRE |
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