In Vitro Response of Polyscias filicifolia (Araliaceae) Shoots to Elicitation with Alarmone–Diadenosine Triphosphate, Methyl Jasmonate, and Salicylic Acid
| Autor: | Katarzyna Sykłowska-Baranek, Marcin Naliwajski, Agnieszka Pietrosiuk, Anita Śliwińska |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Polyscias filicifolia antioxidant activity Cyclopentanes phenolic compounds Acetates 01 natural sciences Antioxidants Article diadenosine 5′ 5‴-P1P3-triphosphate 03 medical and health sciences chemistry.chemical_compound Caffeic Acids oleanolic acid antioxidant enzymes Hydroxybenzoates Caffeic acid Oxylipins Araliaceae lcsh:QH301-705.5 Oleanolic acid elicitation Methyl jasmonate biology Guanosine Pentaphosphate General Medicine Phenolic acid tocopherol biology.organism_classification 030104 developmental biology lcsh:Biology (General) chemistry Biochemistry Shoot Salicylic Acid Dinucleoside Phosphates Salicylic acid 010606 plant biology & botany Alarmone |
| Zdroj: | Cells Cells, Vol 10, Iss 419, p 419 (2021) Volume 10 Issue 2 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10020419 |
| Popis: | The effectiveness of different elicitation variants in combination with alarmone application was studied in shoot cultures of Polyscias filicifolia. The shoots were elicited with 200 µM methyl jasmonate (MeJA) or 50 µM salicylic acid (SA) alone or in combination, and their activity was compared with those treated with the alarmone diadenosine 5′,5‴-P1P3-triphosphate (Ap3A), either alone or in combination with SA and/or MeJA. All treatments resulted in significant stimulation of phenolic acid production (chlorogenic and ferulic acids), as well as oleanolic acid (OA) compared to control, with their highest concentration noted under simultaneous elicitation with SA and MeJA. While the maximum content of caffeic acid was detected after treatment with alarmone alone. In each of the culture variants enhanced antioxidant activity was observed, however the level varied according to the treatment. In addition, the SA, Ap3A and Ap3A+SA variants demonstrated additional peroxidase isoforms, as indicated by Native-PAGE, as well as the highest α-tocopherol content. The highest antioxidant capacity of shoot extracts was correlated with the highest abundance of phenolic compounds and OA. The results indicate that ROS induction appears to participate in the signal transduction following Ap3A treatment. |
| Databáze: | OpenAIRE |
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