Comparison of the Idylla™ Respiratory (IFV-RSV) panel with the GeneXpert Xpert® Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples
| Autor: | Diana Koletzki, W. Laffut, Marc Van Ranst, Els Rousseau, Steven Vissers, Yannick Wouters, Theresa Pattery, Ellen Van Even, Els Keyaerts, Annabel Rector |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male medicine.medical_specialty Adolescent 030106 microbiology Respiratory Syncytial Virus Infections Virus Specimen Handling 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine Nasopharynx Influenza Human medicine Sample Type Humans Sampling (medicine) 030212 general & internal medicine Respiratory system Child Reference standards Aged Retrospective Studies Aged 80 and over GeneXpert MTB/RIF business.industry Infant Newborn Infant Retrospective cohort study Influenza a General Medicine Middle Aged Orthomyxoviridae Infectious Diseases Molecular Diagnostic Techniques Child Preschool Respiratory Syncytial Virus Human Female Nasal Cavity business |
| Zdroj: | Diagnostic microbiology and infectious disease. 94(1) |
| ISSN: | 1879-0070 |
| Popis: | The objective of this study was to compare the performance of the Idylla™ Respiratory (IFV-RSV) panel to the GeneXpert Xpert® Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert® assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla™ assay showed excellent performance compared to the GeneXpert® assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab. |
| Databáze: | OpenAIRE |
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