Analysis of SARS-CoV-2 RNA on surfaces in New York City
| Autor: | Tarun Sharma, Henry W Zhou, Zachary E Snow, Jason Horowitz, Marium Sohail, Vlad Diaconita, Stanley Chang, Alexis S. Kassotis, Saurabh Sudesh, Aliaa H. Abdelhakim, Tongalp H. Tezel, Omar Moussa, Lisa Park, Rabia Karani, Danielle Trief, Angela Y Chang, Qun Zeng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Coronavirus disease 2019 (COVID-19) SARS-CoV-2 business.industry Health Policy Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Public Health Environmental and Occupational Health Columbia university COVID-19 RNA Emergency department Cross-Sectional Studies Nasal Swab Family medicine Transport medium medicine Humans RNA Viral New York City business Pandemics Close contact Research Theme 1: COVID-19 Pandemic |
| Zdroj: | Journal of Global Health |
| ISSN: | 2047-2986 2047-2978 |
| DOI: | 10.7189/jogh.11.05022 |
| Popis: | Background: This study sought to determine the presence of SARS-CoV-2 virus on surfaces that trainees and faculty of an academic eye clinic came into contact with during daily life at the time of the COVID-19 pandemic in New York City. Methods: This cross-sectional analysis involved collection of at least two samples by teams on four different days (November 9, 2020 - December 18, 2020) using sterile swabs (Puritan HydraFlock, Garden Grove, CA). Collection sites were grouped into four zones depending on proximity and amount of time personnel spent there. Samples were transported to the laboratory in transport medium and RNA was extracted using the QIAamp DSP Viral RNA Mini Kit (Qiagen, Germantown, MD). Presence of viral RNA was investigated using the Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipwsich, MA). Results: 834 samples were submitted. Two were positive for SARS-CoV-2 RNA. The first was a sample from a patient bathroom sink handle in the main emergency department. The second was a nasal swab sample from a staff member who had been assigned to collect samples. Prior to this positive result, this asymptomatic staff member had tested positive for COVID-19, had quarantined for two weeks, and had received a negative test. Conclusion: Though COVID-19 is currently widespread in the United States, this study shows that health care personnel working in New York City at the Columbia University Irving Medical Center have a low chance of encountering viral RNA on surfaces they are in close contact with during daily life. |
| Databáze: | OpenAIRE |
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