First full-length sequences of the S gene of European isolates reveal further diversity among turkey coronaviruses

Autor: J. Bertin, D. Toquin, S. Maurel, François-Xavier Briand, Chantal Allée, M. Queguiner, Nicolas Eterradossi, C. Retaux, H. Morvan, L. Ravillion, V. Turblin
Přispěvatelé: Avian and Rabbit Virology Immmunology and Parasitology Reserach Unit (VIPAC), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Coopérative Le Gouessant [Lamballe], MC Vet Conseil, present adress: Ceva Animal Health, Laboratoire de Développement et d'analyse des Côtes d'Armor
Rok vydání: 2011
Předmět:
Turkeys
040301 veterinary sciences
Sequence analysis
Molecular Sequence Data
Biology
medicine.disease_cause
Sensitivity and Specificity
Conserved sequence
0403 veterinary science
03 medical and health sciences
Viral Proteins
Food Animals
Viral Envelope Proteins
Phylogenetics
Genetic variation
medicine
Coronavirus
Turkey
Animals
Amino Acid Sequence
Gene
Conserved Sequence
Phylogeny
030304 developmental biology
Coronavirus
Genetics
0303 health sciences
Membrane Glycoproteins
General Immunology and Microbiology
Phylogenetic tree
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
Life Sciences
Genetic Variation
04 agricultural and veterinary sciences
Sequence Analysis
DNA
Nucleocapsid Proteins
RNA-Dependent RNA Polymerase
Virology
3. Good health
Turkey coronavirus
Spike Glycoprotein
Coronavirus
Enteritis
Transmissible
of Turkeys
RNA
Viral
Animal Science and Zoology
France
Sequence Alignment
Zdroj: Avian Pathology
Avian Pathology, Taylor & Francis, 2011, 40 (02), pp.179-189. ⟨10.1080/03079457.2011.551936⟩
ISSN: 1465-3338
0307-9457
DOI: 10.1080/03079457.2011.551936⟩
Popis: An increasing incidence of enteric disorders clinically suggestive of the poult enteritis complex has been observed in turkeys in France since 2003. Using a newly designed real-time reverse transcriptase-polymerase chain reaction assay specific for the nucleocapsid (N) gene of infectious bronchitis virus (IBV) and turkey coronaviruses (TCoV), coronaviruses were identified in 37% of the intestinal samples collected from diseased turkey flocks. The full-length spike (S) gene of these viruses was amplified, cloned and sequenced from three samples. The French S sequences shared 98% identity at both the nucleotide and amino acid levels, whereas they were at most 65% and 60% identical with North American (NA) TCoV and at most 50% and 37% identical with IBV at the nucleotide and amino acid levels, respectively. Higher divergence with NA TCoV was observed in the S1-encoding domain. Phylogenetic analysis based on the S gene revealed that the newly detected viruses form a sublineage genetically related with, but significantly different from, NA TCoV. Additionally, the RNA-dependent RNA polymerase gene and the N gene, located on the 5' and 3' sides of the S gene in the coronavirus genome, were partially sequenced. Phylogenetic analysis revealed that both the NA TCoV and French TCoV (Fr TCoV) lineages included some IBV relatives, which were however different in the two lineages. This suggested that different recombination events could have played a role in the evolution of the NA and Fr TCoV. The present results provide the first S sequence for a European TCoV. They reveal extensive genetic variation in TCoV and suggest different evolutionary pathways in North America and Europe.
Databáze: OpenAIRE
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