Quantification of active selenols in cells: a selenol-specific recognition europium-switched signal-amplification ICP-MS approach

Autor: Jiaxuan, Zhang, Yang, Zhou, Dongliang, Zuo, Limin, Yang, Xiaowen, Yan, Pingguo, Liu, Qiuquan, Wang
Rok vydání: 2021
Předmět:
Zdroj: Analytical and Bioanalytical Chemistry. 414:257-263
ISSN: 1618-2650
1618-2642
Popis: Selenium (Se) is a mysterious thus tempting element playing a dual bio-chemical function, mainly through selenol, during life processes. Quantification of the selenols is thus of great significance for understanding the biological roles of Se, but remains a big challenge. Herein we report a selenol-specific recognition-mediated and europium (Eu) signal-switched amplification inductively coupled plasma mass spectrometry (ICP-MS) approach for quantifying the free active selenols (act-SeH) in cells. A bifunctional molecule, 2,4-dinitrobenzenesulfonyl-piperidin-4-yl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic europium (DNBS-DOTA-Eu), was designed and synthesized for the specific recognition and highly sensitive quantification of act-SeH via switching Se to more sensitive Eu ICP-MS signals. The limit of detection (LOD, 3σ) of 3.41 pg/mL (22.43 pmol/L), corresponding to the absolute mass LOD of 6.82 ag act-SeH per cell, is almost 25 times lower than 83.76 pg/mL (1.06 nmol/L), 167.52 ag, when monitoring
Databáze: OpenAIRE
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