Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803
| Autor: | Christiane Funk, Yasuo Ishikawa, Wolfgang P. Schröder |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cyanobacteria
Functional analysis biology Photosystem II Synechocystis Photosystem II Protein Complex food and beverages macromolecular substances Cell Biology Plant Science General Medicine Oxygen-evolving complex biology.organism_classification Thylakoids Biochemistry Genome Mutagenesis Insertional Bacterial Proteins Thylakoid Gene family Arabidopsis thaliana Gene Deletion Protein Binding |
| Zdroj: | Photosynthesis Research. 84:257-262 |
| ISSN: | 1573-5079 0166-8595 |
| DOI: | 10.1007/s11120-005-0477-8 |
| Popis: | A recent proteomic analysis of the thylakoid lumen of Arabidopsis thaliana revealed the presence of several PsbP-like proteins, and a homologue to this gene family was detected in the genome of the cyanobacterium Synechocystis sp. PCC 6803 (Schubert M, Petersson UA, Haas BJ, Funk C, Schröder WP, Kieselbach T (2002) J Biol Chem 277, 8354-8365). Using a peptide-directed antibody against this cyanobacterial PsbP-like protein (sll1418) we could show that it was localized in the thylakoid membrane and associated with Photosystem II. While salt washes did not remove the PsbP-like protein from the thylakoid membrane, it was partially lost during the detergent-based isolation of PSII membrane fractions. In total cell extracts this protein is present in the same amount as the extrinsic PsbO protein. We did not see any significant functional difference between the wild-type and a PsbP-like insertion mutant. |
| Databáze: | OpenAIRE |
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