Transcriptome analysis of soybean (Glycine max) root genes differentially expressed in rhizobial, arbuscular mycorrhizal, and dual symbiosis
| Autor: | Yurie Sugimoto, Mitsuru Ueno, Motoaki Seki, Maho Tanaka, Junko Ishida, Natsuko Ogiwara, Yasushi Totoki, Tomomitsu Kaji, Masatoshi Sonoda, Kazuo Shinozaki, Kazunori Sakamoto, Akihiro Matsui |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Root nodule Plant Science Genes Plant 01 natural sciences Plant Roots Rhizobia Transcriptome 03 medical and health sciences Symbiosis Gene Expression Regulation Plant Mycorrhizae MYB Gene Oligonucleotide Array Sequence Analysis Genetics biology Host (biology) Gene Expression Profiling fungi food and beverages biology.organism_classification 030104 developmental biology Soybeans DNA microarray Root Nodules Plant 010606 plant biology & botany Rhizobium |
| Zdroj: | Journal of plant research. 132(4) |
| ISSN: | 1618-0860 |
| Popis: | Soybean (Glycine max) roots establish associations with nodule-inducing rhizobia and arbuscular mycorrhizal (AM) fungi. Both rhizobia and AM fungi have been shown to affect the activity of and colonization by the other, and their interactions can be detected within host plants. Here, we report the transcription profiles of genes differentially expressed in soybean roots in the presence of rhizobial, AM, or rhizobial–AM dual symbiosis, compared with those in control (uninoculated) roots. Following inoculation, soybean plants were grown in a glasshouse for 6 weeks; thereafter their root transcriptomes were analyzed using an oligo DNA microarray. Among the four treatments, the root nodule number and host plant growth were highest in plants with dual symbiosis. We observed that the expression of 187, 441, and 548 host genes was up-regulated and 119, 1,439, and 1,298 host genes were down-regulated during rhizobial, AM, and dual symbiosis, respectively. The expression of 34 host genes was up-regulated in each of the three symbioses. These 34 genes encoded several membrane transporters, type 1 metallothionein, and transcription factors in the MYB and bHLH families. We identified 56 host genes that were specifically up-regulated during dual symbiosis. These genes encoded several nodulin proteins, phenylpropanoid metabolism-related proteins, and carbonic anhydrase. The nodulin genes up-regulated by the AM fungal colonization probably led to the observed increases in root nodule number and host plant growth. Some other nodulin genes were down-regulated specifically during AM symbiosis. Based on the results above, we suggest that the contribution of AM fungal colonization is crucial to biological N2-fixation and host growth in soybean with rhizobial-AM dual symbiosis. |
| Databáze: | OpenAIRE |
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