Virulence characterization of Klebsiella pneumoniae and its relation with ESBL and AmpC beta-lactamase associated resistance
| Autor: | Mohammad Ahangarzadeh Rezaee, Tahereh Pirzadeh, Arezoo Noie Oskouie, Akbar Hasani, Alka Hasani, Elghar Soltani, Ehsan Binesh, Mahin Ahangar Oskouee |
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| Rok vydání: | 2020 |
| Předmět: |
Klebsiella pneumoniae
Virulence Antibiotic resistance Extended spectrum beta-lactamase AmpC β-lactamase Microbiology (medical) medicine.drug_class Antibiotics lcsh:QR1-502 AmpC beta-lactamase biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses biology.organism_classification Microbiology lcsh:Microbiology law.invention law polycyclic compounds medicine bacteria Agar diffusion test Gene Polymerase chain reaction |
| Zdroj: | Iranian Journal of Microbiology, Vol 12, Iss 2 (2020) |
| ISSN: | 2008-4447 2008-3289 |
| Popis: | Background and Objectives: Trend analysis reveals that Klebsiella pneumoniae has witnessed a steep enhancement in the antibiotic resistance and virulence over the last few decades. The present investigation aimed at a comprehensive approach investigating antibiotic susceptibility including, extended spectrum beta-lactamase (ESBL) and AmpC β-lactamase (AmpC) resistance and the prevalence of virulence genes among the K. pneumoniae isolates. Materials and Methods: Sixty-one K. pneumoniae isolates were obtained from various clinical infections. Antimicrobial susceptibility was performed by disk diffusion method. The Mast® D68C test detected the presence of ESBLs and AmpCs phenotypically, and later presence of ESBL and AmpC genes was observed by polymerase chain reaction (PCR). Multi- plex-PCR was performed to investigate various virulence genes. CMY-2 Results: Amongst 61 K. pneumoniae isolates, 59% were observed as ESBL and 14.7% as AmpC producers. All ESBL CTX-M-15 producers were positive for bla CTX-M-15 , while bla CTX-M-14 was observed in 54.1% isolates. The frequency of AmpC genes was CMY-2 as follows: bla CMY-2 (60.7%) and bla DHA-1 (34.4%). The most frequent virulence genes were those encoding enterobactin and DHA-1 lipopolysaccharide. Presence of mrkD was associated with bla CMY-2 DHA-1 gene, while bla significantly (p≤0.05) correlated DHA-1 with the presence of iutA and rmpA virulence genes. bla positive isolates had urine as a significant source, while bla positive isolates were mainly collected from wound exudates (p≤0.05). Conclusion: Our results highlight that ESBL and AmpC production along with a plethora of virulence trait on K. pneumoni- ae should be adequately considered to assess its pathogenesis and antibiotic resistance. |
| Databáze: | OpenAIRE |
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