Enhanced serum levels of soluble HLA class I molecules are induced by treatment with recombinant interferon-gamma (IFN-γ)
| Autor: | Walter E. Aulitzky, H. Grosse-Wilde, Guenther Gastl, W. E. Aulitzky, Marco J Herold, U. Westhoff, H. Tilg, C. Huber |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Immunology Human leukocyte antigen law.invention Interferon-gamma law Internal medicine medicine Humans Immunology and Allergy Interferon gamma Carcinoma Renal Cell Kidney Dose-Response Relationship Drug Beta-2 microglobulin business.industry Histocompatibility Antigens Class I Radioimmunoassay Recombinant Interferon Gamma Middle Aged Recombinant Proteins medicine.anatomical_structure Cytokine Endocrinology Solubility Recombinant DNA Drug Evaluation beta 2-Microglobulin business Research Article medicine.drug |
| Zdroj: | Clinical and Experimental Immunology. 86:236-239 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.1991.tb05802.x |
| Popis: | SUMMARY In order to investigate serum levels of soluble HLA class I antigens after single injection of various doses of recombinant IFN-γ (rIFN-γ) and to correlate the changes observed to beta-2-microglobulin serum levels, we studied five patients with metastasizing renal cell carcinoma. Each patient received three treatment cycles of 10, 100 and 500 μg rIFN-γ three times at weekly intervals. The treatment cycles were separated by a therapy-free interval of 2 weeks. The order of dose levels was randomly assigned to each patient. Serum levels of soluble HLA class I proteins were measured by an ELISA in samples drawn immediately before and 4, 24, 48, 72 and 168 h after each administration of rIFN-γ. Beta-2-microglobulin was assessed in parallel using a commercially available radioimmunoassay. Significant induction of soluble HLA class I protein serum levels was observed after treatment with 100 and 500 μg rIFN-γ. The increments peaked after 2–4 days and remained elevated for up to more than 7 days. A significant correlation between increments of soluble HLA class I proteins and beta-2-microglobulin was observed. We conclude that measurement of soluble HLA serum levels is practical for monitoring induction of HLA class I synthesis in patients treated with rlFN-γ. The correlation observed between induction of beta-2-microglobulin and soluble HLA class 1 proteins indicates that measurement of beta-2-microglobulin might be sufficient for the biological response monitoring in clinical studies. |
| Databáze: | OpenAIRE |
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