Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay
| Autor: | James A. Kellogg, J W Seiple, E S Stroll |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Fluorescent Antibody Technique Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay Cervix Uteri Immunofluorescence medicine.disease_cause Sensitivity and Specificity Antigen Pregnancy medicine Humans Chlamydiaceae Direct fluorescent antibody chemistry.chemical_classification Antigens Bacterial medicine.diagnostic_test biology Chlamydia Infections biology.organism_classification Virology Enzyme chemistry Evaluation Studies as Topic Chlamydiales Reagent Female Research Article |
| Zdroj: | Journal of Clinical Microbiology. 31:1646-1647 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.31.6.1646-1647.1993 |
| Popis: | Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed with the direct fluorescent-antibody test (Syva) from among those 66 Chlamydiazyme-negative specimens which had A492s that ranged from 0.030 to the cutoff and that could be blocked by > or = 50% with the blocking reagent. |
| Databáze: | OpenAIRE |
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