Virulence potential of Listeria monocytogenes strains recovered from pigs in Spain

Autor: Jaime Gómez-Laguna, Javier Pizarro-Cerdá, Juan J. Quereda, Jazmín Meza-Torres, Fernando Cardoso-Toset
Přispěvatelé: Universidad de Córdoba = University of Córdoba [Córdoba], Centro de investigación agroaliementaria - Food Research Center [Córdoba, Spain] (CICAP), Yersinia, Institut Pasteur [Paris] (IP), Centre National de Référence de la Peste et autres Yersinioses - National Reference Center Plague and Yersinioses (CNR), Universidad Cardenal Herrera-CEU (CEU-UCH), This study was supported by the Agency for Innovation and Development of Andalucía (IDEA, project reference 351504/550859), by the University of Córdoba Research Program (Spain), by the Andalusian FEDER Operational Funding Program, by Generalitat Valenciana (project reference GV/2018/A/183) and by the Spanish Ministry of Science and Innovation (project reference PID2019- 110764RA- I00). JG- L is supported by a ’Ramón y Cajal’ contract of the Spanish Ministry of Economy and Competitiveness (RYC-2014-16735). JJQ is supported by a ’Ramón y Cajal’ contract of the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985- I). The funding bodies had no role in the design of the study, in the collection, analysis and interpretation of data, and in writing the manuscript.
Rok vydání: 2020
Předmět:
Zdroj: Veterinary Record
Veterinary Record, 2020, 187 (11), pp.e101-e101. ⟨10.1136/vr.105945⟩
ISSN: 0042-4900
DOI: 10.1136/vr.105945
Popis: International audience; Background: Listeria monocytogenes is a foodborne bacterial pathogen that causes listeriosis, an infectious disease of animals and humans, with pigs acting as asymptomatic reservoirs. In August 2019 a listeriosis outbreak associated with the consumption of pork meat caused 222 human cases in Spain. Determining the diversity as well as the virulence potential of strains from pig origin is important for public health concerns. Methods: The behavior of twenty-three L. monocytogenes strains recovered from pig tonsils, meat and skin was compared by studying: (1) Internalin A (InlA), Internalin B (InlB), Listeriolysin O (LLO), Actin assembly-inducing protein (ActA) and PrfA expression levels, and (2) invasion and intracellular growth in eukaryotic cells. Results: Marked differences were found in the expression of the selected virulence factors and the invasion and intracellular replication phenotypes of the L. monocytogenes strains. Strains obtained from meat samples and belonging to ST 1/2a did not have InlA anchored to the peptidoglycan. Some strains expressed higher levels of the studied virulence factors and invaded and replicated intracellularly more efficiently than an epidemic L. monocytogenes reference strain (F2365). Conclusion: This study demonstrates the presence of virulent L. monocytogenes strains in pigs, with valuable implications in veterinary medicine and food safety.
Databáze: OpenAIRE
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