Specific induction of double negative B cells during protective and pathogenic immune responses

Autor: Gisela Gabernet, Ulf Ziemann, Bernhard Hemmer, Sven Nahnsen, Simon Heumos, Greg Owens, Martin Irmler, Gildas Lepennetier, Miriam Kaminski, Jeffrey Bennett, Markus C. Kowarik, Christoph Ruschil, Zsuzsanna Hrasko, Johannes Beckers
Rok vydání: 2020
Předmět:
Male
double negative B cells
Antibodies
Viral
Lymphocyte Activation
autoimmune disorders
Transcriptome
Immunogenicity
Vaccine
Autoimmune Disorders
B Cells
Double Negative B Cells
Influenza Vaccination
Neuromyelitis Optica Spectrum Disorder
Tbe Vaccination
Vaccination
Original Research
Aged
80 and over
B-Lymphocytes
education.field_of_study
biology
neuromyelitis optica spectrum disorder
Middle Aged
influenza vaccination
Phenotype
medicine.anatomical_structure
Influenza Vaccines
Female
Antibody
Encephalitis
Adult
Adolescent
Immunology
Antigens
CD19
Population
Naive B cell
Communicable Diseases
CD19
TBE vaccination
Encephalitis Viruses
Tick-Borne
Young Adult
Immune system
medicine
Humans
ddc:610
education
B cell
Aged
Cell Proliferation
Inflammation
B cells
Viral Vaccines
Antigens
CD20
medicine.disease
Immunity
Humoral
Tumor Necrosis Factor Receptor Superfamily
Member 7
Case-Control Studies
biology.protein
Zdroj: Europe PubMed Central
Frontiers in Immunology
Front. Immunol. 11:606338 (2020)
Popis: 1AbstractDouble negative (DN) (CD19+CD20lowCD27−IgD−) B cells are expanded in patients with autoimmune and infectious diseases; however their role in the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood B cell subsets, we observed an inflated DN B cell population in patients with variety of active inflammatory conditions: myasthenia gravis, Guillain-Barré syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, and rheumatic disorders. Furthermore, we were able to induce DN B cells in healthy subjects following vaccination against influenza and tick borne encephalitis virus. Transcriptome analysis revealed a gene expression profile in DN B cells that clustered with naïve B cells, memory B cells, and plasmablasts. Immunoglobulin VH transcriptome sequencing and analysis of recombinant antibodies revealed clonal expansion of DN B cells, that were targeted against the vaccine antigen. Our study suggests that DN B cells are expanded in multiple inflammatory neurologic diseases and represent an inducible B cell population that responds to antigenic stimulation, possibly through an extra-follicular maturation pathway.
Databáze: OpenAIRE
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