Monitoring of SARS-CoV-2 antibodies using dried blood spot for at-home collection
| Autor: | Peyton K. Miesse, Bradley B. Collier, Russell P. Grant |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Multidisciplinary biology medicine.diagnostic_test business.industry SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 Serum samples Antibodies Viral Gastroenterology Serology Dried blood spot COVID-19 Serological Testing Vaccination Internal medicine Immunoassay medicine biology.protein Humans Dried Blood Spot Testing Antibody business Expansive |
| Zdroj: | Scientific reports. 12(1) |
| ISSN: | 2045-2322 |
| Popis: | The utilization of vaccines to fight the spread of SARS-CoV-2 has led to a growing need for expansive serological testing. To address this, an EUA approved immunoassay for detection of antibodies to SARS-CoV-2 in venous serum samples was investigated for use with dried blood spot (DBS) samples. Results from self-collected DBS samples demonstrated a 98.1% categorical agreement to venous serum with a correlation (R) of 0.9600 while professionally collected DBS samples demonstrated a categorical agreement of 100.0% with a correlation of 0.9888 to venous serum. Additional studies were performed to stress different aspects of at-home DBS collection, including shipping stability, effects of interferences, and other sample-specific robustness studies. These studies demonstrated a categorical agreement of at least 95.0% and a mean bias less than ± 20.0%. Furthermore, the ability to track antibody levels following vaccination with the BioNTech/Pfizer vaccine was demonstrated with serial self-collected DBS samples from pre-dose (Day 0) out to 19 weeks. |
| Databáze: | OpenAIRE |
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