ELS1, a novel MATE transporter related to leaf senescence and iron homeostasis in Arabidopsis thaliana
| Autor: | Chongzhen Qian, Erlong Liu, Wei Zhang, Xiaochun Guo, Kaili Mao, Ni Chen, Changjun Mu, Heng Liu, Zhenyu Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Senescence Aging Organic Cation Transport Proteins Iron Mutant Arabidopsis Biophysics Biology medicine.disease_cause 01 natural sciences Biochemistry 03 medical and health sciences medicine Homeostasis Arabidopsis thaliana Molecular Biology Gene Genetics Mutation Arabidopsis Proteins Membrane Transport Proteins food and beverages Transporter Cell Biology Plants Genetically Modified biology.organism_classification Phenotype Cell biology Plant Leaves 030104 developmental biology 010606 plant biology & botany |
| Zdroj: | Biochemical and Biophysical Research Communications. 476:319-325 |
| ISSN: | 0006-291X |
| Popis: | The multidrug and toxic compound extrusion (MATE) transporters mediate the coupled exchange of organic substrates and monovalent cations have been recently implicated in various plant biological activities. In this work, we isolated a dominant mutant from an Arabidopsis activation-tagging mutant pool. This mutant exhibits pleiotropic phenotype including early flowering, dwarf and bushy architecture, minified lateral organs and early leaf senescence, and is therefore designated early leaf senescence 1-Dominaint (els1-D). Genotyping assays showed that els1-D is a gain-of-function mutant of a novel MATE transporter gene, ELS1, which encodes a close homolog of the previously reported ADP1, BCD1 and DTX50. Further investigations revealed that the overexpression of ELS1 reduces iron content in els1-D, and the accelerated senescence of the detached els1-D leaves can be recovered by exogenous iron supply. In addition, we also found that ELS1 is an iron responsive gene. Based on these findings, we proposed that ELS1 is related to leaf senescence and iron homeostasis in Arabidopsis. |
| Databáze: | OpenAIRE |
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