Histamine stimulates matrix metalloproteinase-3 and -13 production by human articular chondrocytes in vitro
| Autor: | D E Woolley, Lynne C Tetlow |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Matrix Metalloproteinase 3
Concise Report Immunology Enzyme-Linked Immunosorbent Assay Biology Matrix metalloproteinase General Biochemistry Genetics and Molecular Biology Stromelysin 1 Chondrocyte chemistry.chemical_compound Chondrocytes Rheumatology Matrix Metalloproteinase 13 medicine Humans Immunology and Allergy Collagenases Molecular biology In vitro medicine.anatomical_structure chemistry Cell culture Collagenase Histamine medicine.drug |
| Zdroj: | Annals of the Rheumatic Diseases. 61:737-740 |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.61.8.737 |
| Popis: | To determine the effects of histamine on matrix metalloproteinase (MMP) production by human articular chondrocytes (HAC) in vitro.Conditioned culture medium from HAC cultures incubated with and without 20 microM histamine was assayed by enzymne linked immunosorbent assay (ELISA) for MMP-1, MMP-8, MMP-13 (collagenases 1, 2, and 3, respectively) and MMP-3 (stromelysin). Monolayer cultures of HAC were also immunostained for MMP-13 and MMP-3.The HAC cultures showed a significant increase in MMP-13 and MMP-3 production (2.2- and 1.9-fold, respectively) after treatment with 20 microM histamine for 24 hours, but MMP-1 and MMP-8 were unaffected. All cultures showed MMP-13 and MMP-3 detectable by immunolocalisation. MMP-3 was the more prominent enzyme as shown by both ELISA and immunolocalisation techniques.Histamine exposure increased both MMP-13 and MMP-3 production by HAC in vitro, thereby suggesting a pathophysiological role in the chondrocytic phenotype associated with degenerative changes in osteoarthritis. |
| Databáze: | OpenAIRE |
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