Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen
| Autor: | Sakata, Toshiya, Ihara, Masaki, Makino, Izumi, Miyahara, Yuji, Ueda, Hiroshi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Bisphenol A
Transistors Electronic Immunoglobulin Variable Region Analytical chemistry Field effect Maltose-Binding Proteins Analytical Chemistry law.invention chemistry.chemical_compound Phenols Isothiocyanates law Antigens Benzhydryl Compounds Electrodes Edetic Acid Immunoassay Detection limit Molecular mass Transistor Charge density Electrochemical Techniques chemistry Electrode Immunoglobulin Light Chains Field-effect transistor Carrier Proteins Immunoglobulin Heavy Chains |
| Zdroj: | Anal. Chem.. 81(No. 18) |
| Popis: | In this study, we proposed a new detection method, open sandwich-based immuno-field effect transistor (OS-FET) for label-free and noncompetitive detection of low molecular weight antigen. The principle of OS-FET is based on the detection of intrinsic molecular charges caused by the small antigen-dependent interchain interaction of separated V(L) and V(H) chains from a single antibody variable region using the field effect. Introducing V(H) chain and small antigen bisphenol A into the OS-FET with the immobilized V(L) chain on the gate, we could detect electrically and directly the binding of bisphenol A by V(H) and V(L) chains. Although the detection limit of OS-FET was 1 nM to detect bisphenol A for the standard deviation of control signal, the addition of isothiocyanobenzyl-EDTA with negative charges to the V(L) chain enhanced the detection limit to 1 pM. We could directly transduce the charge density changes based on the capture of target on the gate into the electrical signals using the OS-FET. The platform based on the FETs is suitable for a label-free, noncompetitive, and quantitative detection system for small antigen analysis in environmental, food, and clinical research. |
| Databáze: | OpenAIRE |
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